摘要
目的:观察疏肝解郁经典名方柴胡疏肝散对Aβ1-40诱导的肝郁证阿尔茨海默病(Alzheimerdisease,AD)病理模型大鼠行为学和海马神经递质等指标的影响,以期阐明柴胡疏肝散对肝郁证AD的治疗作用及其基本机制。方法:雄性健康SD大鼠随机分为6组:正常对照组、假手术组对照组、AD模型对照组、西药安理申对照组、柴胡疏肝散高、低剂量组,每组l5只。束缚法连续3周,建立实验性大鼠肝郁模型;肝郁模型大鼠的海马内注射Aβ1-40建立肝郁型AD病证结合模型。Morris水迷宫试验观测实验各组行为学指标的变化,HPLC-ECD技术检测各组大鼠脑组织神经递质NE、DA和5-HT含量。结果:糖水偏好实验结果显示,与正常组相比,模型组大鼠糖水偏好度明显降低(P<0.01);与模型组比较,安理申组和柴胡疏肝散组大鼠糖水偏好度明显提高(P<0.01),柴胡疏肝散高剂量组较安理申组提高更明显(P<0.05)。Morris水迷宫试验结果发现,模型组大鼠寻找平台时间及逃避潜伏期较正常组明显延长(P<0.01);与模型组比较,安理申组和柴胡疏肝散组大鼠寻找平台时间及逃避潜伏期明显缩短(P<0.01),安理申组和中药高剂量组相比无明显差异(P>0.05)。大鼠海马组织神经递质的含量监测结果显示,模型组大鼠海马组织NA、5-HT和DA水平较正常组显著降低(P<0.01);与模型组比较,安理申组和柴胡疏肝散组大鼠海马组织NA、5-HT和DA水平明显升高(P<0.01)。结论:Aβ1-40结合束缚法可成功建立病症结合的肝郁证AD动物模型;柴胡疏肝散能增加动物对糖水的偏好度,可显著改善模型动物的抑郁状态与记忆和学习能力,其机制可能与其明显提高大鼠海马内的神经递质NE、DA和5-HT水平有关。
Objective To observe the effects of relieving the depressed liver classics prescription-ChaihuShugan San（CHSGS） on behavior and hippocampal monoamine neurotransmitter and other indicators in liver qidepression senile dementia rat pathology model induced by Aβ1-40,in order to clarify the therapeutical effect ofCHSGS on liver qi depression Alzheimer＇s disease（AD） and its underlying mechanism.Methods Male SDrats were randomly divided into six groups：normal control group,sham control group,AD model group,western medicineAricept control group,CHSGS high and low dose groups,with l5 rats in each group.Experimental liver qi depressionmodel rats were established by binding method for continuous 3 weeks.Then the model rats were injected with Aβ1-40in hippocampus to establish liver qi depression syndrome type AD models.Morris water maze test was used to observe thechanges of behavioral indicators.The contents of the rat brain neurotransmitters NE,DA and5-HT were detected byHPLC-ECD technique.Results Sucrose preference test results showed that the degree of sugar preference ofmodel group was significantly lower than that of the normal group（P0.01）;Compared with the model group,the degrees of sugar preference of Aricept group and CHSGS group were obviously increased（P0.01）,and the degreeof sugar preferences of CHSGS high-dose group was improved more significantly than that of the Aricept group（P0.05）.The results of Morris water maze test showed that the time of rat finding the platform and escapelatency period of the model group were significantly longer than those of the normal group（P0.01）.Comparedwith the model group,the time of rat finding the platform and escape latency period of Aricept group andCHSGS group were significantly shorter（P0.01）.There was no obvious difference of the time of rat finding theplatform and escape latency period between the CHSGS high-dose group and the Aricept group（P0.05）.Hippocampus neurotransmitters monitoring test results showed that the NA,5-HT and DA contents in the AD modelgroup hippocampus tissues were notably lower than those in the normal group（P0.01）.Compared with the ADmodel group,the levels of NA,5-HT and DA of Aricept group and CHSGS group hippocampus tissues wereincreased significantly（P 0.01）.Conclusion Hippocampus injection with Aβ1-40 combining with bindingmethod could successfully establish the liver qi depression AD animal model.CHSGS could increase the animal＇ssugar preference,significantly improve the state of depression,and enhance the memory and learning ability.The mechanism might be related that CHSGS could improve the levels of hippocampus neurotransmitters NE,DA and 5-HT.
出处
《深圳中西医结合杂志》
2013年第3期129-134,共6页
Shenzhen Journal of Integrated Traditional Chinese and Western Medicine
基金
国家自然科学基金资助项目(30973779)
关键词
柴胡疏肝散
肝郁证
阿尔茨海默病
学习记忆能力
神经递质
Alzheimer＇s disease（AD）
Chaihu Shugan San（CHSGS）
Liver qi depression
Learning and memory ability
Neurotransmitters
