摘要
目的:建立枳术颗粒5种黄酮类物质含量控制方法。方法:采用高效液相色谱法在同一色谱条件下测定枳术颗粒中柚皮苷、橙皮苷、新橙皮苷、柚皮素、橙皮素含量。色谱条件:流动相乙腈-0.1%磷酸水梯度洗脱;检测波长为285 nm。结果:柚皮苷进样量在22.655～591.734μg范围内呈良好线性关系,相关系数r2=0.9994;橙皮苷进样量在2.981～71.532μg范围内呈良好线性关系,相关系数r2=0.9997;新橙皮苷进样量在22.152～531.648μg范围内呈良好线性关系,相关系数r2=0.9994;柚皮素在3.015～72.378μg范围内呈良好线性关系,相关系数r2=0.9997;橙皮素在2.868～68.85μg范围内呈良好线性关系,相关系数r2=0.9990;5个成分的平均回收率分别为99.9%、100.4%、98.6%、99.9%和100.0%(RSD%<3%)。结论:本方法准确可靠,可准确测定枳术颗粒中5种黄酮类物质的含量。
Objective：To creat a control method for the contents of five flavonoids in Zhizhu granule Methods：The contents of naringin,hesperidin,new hesperidin,naringenin and hesperetin in Zhizhu granule were determined under the same chromatographic condition with HPLC.Chromatographic condition：mobile phase,acetonitrile-0.1% phosphoric acid water for gradient elution;detection wavelength,285 nm.Results：Naringin had a good liner relationship with sample quantity in 22.655～591.734 μg,related coefficient was 0.9994;hesperidin had a good liner relationship with sample quantity in 2.981～71.532 μg,related coefficient was 0.9997;new hesperidin had a good liner relationship with sample quantity in 22.152～531.648 μg,related coefficient was 0.9994;naringenin had a good liner relationship with sample quantity in 3.015～ 72.378 μg,related coefficient was 0.9997;hesperetin had a good liner relationship with sample quantity in 2.868 ～68.85 μg,related coefficient was 0.9990;The respectively average recovery rate of the five was 99.9%,100.4%,98.6%,99.9% and 100.0%（RSD%3%）.Conclusion：This method is accurate,reliable and can accurately measure the contents of five flavonoids in Zhizhu granule.
出处
《药学与临床研究》
2013年第3期232-234,共3页
Pharmaceutical and Clinical Research
关键词
枳术颗粒
柚皮苷
橙皮苷
新橙皮苷
柚皮素
橙皮素
测定方法
Zhizhu granule
Naringin
Hesperidin
New hesperidin
Naringenin
Hesperetin
Determination method
