摘要
利用1.5%NaCl溶液对来源不同的293份小麦种质进行盐胁迫处理,调查各种质的芽期相对盐害指数;从芽期耐盐性鉴定中筛选出16份芽期相对盐害指数小于20%的高耐盐种质和42份芽期相对盐害指数介于20%~40%的耐盐种质;选择芽期相对耐盐指数小于40%的24份小麦资源,进行苗期不同盐浓度胁迫鉴定,测量不同处理的相对根长及苗高,根据芽期相对盐害指数及苗期相对根长计算24份小麦种质资源的耐盐隶属函数综合值。苗期鉴定表明,1.0%NaCl盐浓度为合适的筛选浓度、相对根长为适合的苗期耐盐性鉴定指标,对24份小麦种质资源的耐盐隶属函数综合值进行聚类分析,将其分为3大类:Marmin-Minhardi×H44-Minturki等10份综合耐盐性高于山融3号的为第Ⅰ类,早小麦等综合耐盐性与山融3号相当的10份小麦为第Ⅱ类,洋麦1等4份综合耐盐性低于山融3号的种质为第Ⅲ类。来源不同的小麦种质芽期综合耐盐性大小依次为:地方品种和选育品种>国外引进品种>高代品系;Marmin-Minhardi×H44-Minturki、圆白壳小麦、花树蒲、和尚麦和顶籽锤5份耐盐种质,可作为小麦耐盐研究的新种质。
293 accessions of wheat germplasms were treated with 1.5% NaCl at germination stage to screen for salinity tolerance in terms of the relative salt injury rate,16 high salt-tolerant accessions with the index of salt injury rate less than 20% and 42 salt-tolerant accessions with the index of salt injury ranged 20%-40% were found.24 accessions with relative salt injury rate below 40% were treated with different NaCl concentrations at seedling stage to get the relative growth rate.The concentration of 1.0% NaCl was suitable for screening the wheat salt-tolerance at seedling stage.Then a cluster analysis for the membership function integrated values with relative salt injury rate and root relative growth rate was conducted with the SPSS.24 wheat varieties were divided into 3 groups,10 varieties with better salinity tolerance than check variety Shanrong 3 were in group Ⅰ,another 10 varieties with similar salinity tolerance with Shanrong 3 were in group Ⅱ,while group Ⅲ contained 4 other varieties.The salinity tolerance in germplasm accessions were bred varieties and landraces > introduced varieties > advanced lines according to their origins.Marmin-Minhardi × H44-Minturki,Yuanbaike,Heshangmai,Huashupu,and Dingzichui were newly evaluated with salinity tolerance and might be useful in wheat improvement for salinity tolerance.
出处
《植物遗传资源学报》
CAS
CSCD
北大核心
2013年第4期620-626,共7页
Journal of Plant Genetic Resources
基金
江苏省农业科技自主创新资金项目(CX(12)2024)
科技基础性工作专项(2007FY110500-06)
农业部作物种质资源保护专项(NB2010-2130135-25-02-6)
关键词
小麦种质
芽期
苗期
耐盐
wheat germplasm
germination stage
seedling stage
salinity tolerance