摘要
目的:研究胃癌患者外周血中CD71/44、CD133/3调节性T细胞(Tregulatory cells,Treg)的表达情况及与胃癌临床病理的关系。方法:胃癌患者外周血及正常对照组中Treg的表达情况以流式细胞仪进行检测,结合胃癌患者临床病理情况分析。结果:正常对照组外周血Treg表达率为CD71+/CD44+(11.62±3.26)%、CD133-/CD3+(24.53±9.86)%,胃癌患者外周血Treg表达率为CD71+/CD44+(15.6±7.56)%、CD133-/CD3+(28.64±10.98)%。患者均较正常对照组显著增高(P<0.05)。胃癌患者外周血Treg的表达与胃癌转移率和TNM分期呈负相关,胃癌转移阳性和TNM分期越晚,Treg表达率越高。结论:胃癌患者外周血调节性T细胞(Treg)的表达较正常对照组显著变化,并与临床病理存在一定相关性,有待进一步研究。
Objective ： To evaluate the expressions of CD71/CD44 and CD133/3 T cells in peripheral blood from patients with gastric cancer, and study the correlation with clinical pathology of gastric cancer. Methods： The Treg cell expression level was detected in peripheral blood of gastric cancer patients and normal controls by flowcytometry. Results：The Treg expression levels were analyzed with clinical pathological information. The expression levels of Treg cells in control group were CD71 ＋/CD44 ＋ （ 11.62 ± 3.26） % , CD133 -/CD3 ＋ （24.53 ± 9.86） %, much lower than those in gastric cancer patients, CD71 ＋/CD44 ＋ （ 15.6 ± 7.56） % , CD133 -/CD3 ＋ （28.64 ± 10. 98 ） %. The expression level of Treg ceils in peripheral blood of gastric cancer patients was correlated negatively with lymph node metastasis and TNM classification. Conclusion：Treg ceils expression levels rose significantly in gastric cancer tissues and correlated with clinical information, indicating the immune state of gastric cancer patients.
出处
《中国卫生检验杂志》
北大核心
2013年第6期1505-1506,1509,共3页
Chinese Journal of Health Laboratory Technology
