摘要
目的观察不同厂家生产的2种棘球绦虫IgG抗体检测试剂盒的检测效果。方法采用2种不同厂家生产的棘球绦虫IgG抗体检测试剂盒检测相同数量的棘球蚴病人、正常人血清,以及卫氏并殖吸虫、华支睾吸虫、日本血吸虫和猪囊尾蚴病人血清,统计2种方法的特异性、敏感性和交叉反应性。结果2个厂家生产的棘球绦虫IgG抗体检测试剂盒的特异性和敏感性均为100%。深圳康百得公司生产的试剂盒与卫氏并殖吸虫病、华支睾吸虫病、日本血吸虫病和猪囊尾蚴病的交叉反应率分别为25.00%、26.09%、10.00%和87.50%;珠海海泰公司生产的试剂盒的交叉反应率分别为5.00%、13.04%、20.00%和93.75%。2种试剂盒间,卫氏并殖吸虫病检出率差异有统计学意义(P<0.05),其他检出率差异均无统计学意义(P均>0.05)。结论2个厂家生产的棘球绦虫IgG抗体检测试剂盒均具有敏感性高、特异性强、简便快速等优点;但需进一步解决与其他寄生虫病的交叉反应问题,以提高早期诊断的准确性。
Objective To compare the effects of two ELISA kits on IgG antibody detection of human Echinococcus granulo sus.Methods A Total of 134 sera of patients with echinococcosis,paragonimiasis westermani,clonorchiasis sinensis,schistoso miasis japonica,and cysticercosis cellulosae,and normal persons were detected by two IgG ELISA kits produced by different com panies.Furthermore,the specificity,sensitivity and cross reactivity were counted and analyzed statistically.Results The sensi tivity and specificity were extremely high of the two kits as 100.00%.The cross-reactivity rates were 25.00%（paragonimiasis wes termani）,26.09%（clonorchiasis sinensis）,10.00%（schistosomiasis japonica）,and 87.5%（cysticercosis）,respectively,by us ing the kit produced by the Combined Company in Shenzhen;the cross-reactivity rates were 5.00%（paragonimiasis westermani）,13.04%（clonorchiasis sinensis）,20.00%（schistosomiasis japonica）,and 93.75%（cysticercosis）respectively,by using the kit produced by Haitai Company in Zhuhai.In addition,there was a significant difference of Paragonimus westermani detection（P 0.05）,but the rests had no statistically significant differences（all P 0.05）between the two kits.Conclusion Both ELISA kits on IgG antibody detection of human Echinococcus granulosus have the advantages of a high sensitivity,specificity,convenience and high-speed.However,it is also in urgent need to further solve the cross-reactivity of Echinococcus granulosus with other para sites,in order to improve the accuracy of early diagnosis.
出处
《中国血吸虫病防治杂志》
CAS
CSCD
2013年第3期284-286,共3页
Chinese Journal of Schistosomiasis Control
基金
中国博士后科学基金(2012M520353)
上海市博士后科学基金(12R21416500)
国家重大科技专项(2012ZX10004-220
2008ZX10004-011)
国家科技支撑计划(2008BAI56B03)
关键词
棘球绦虫
IGG抗体
酶联免疫吸附试验
试剂盒
交叉反应
Echinococcus granulosus
IgG antibody
Enzyme-linked immunosorbent assay（ELISA）
Kit
Cross-reaction
