摘要
目的了解流感流行趋势和病原学特征,为流感防治提供科学依据。方法收集郴州市流感监测哨点医院2010-2012年流感样病例的基本信息,并采集鼻、咽拭子,以狗肾传代细胞（MDCK）进行分离培养,采用血凝（HA）及血凝抑制（HI）方法进行病毒分离培养及分型鉴定。结果 2010-2012年共监测流感样病例18 265例,流感样病例主要集中于0~14岁组,占71.7%,男女性别比为1.54：1,采集咽拭子样本3 859份,经病毒分离和分型鉴定的流感病毒242株,总分离率6.3%,其3年的分离率分别为4.8%、7.4%、7.8%,分离到了BV、BY、H3、新甲H1N1四种亚型,但3年均以BV亚型为主。发现2株BY型毒株与疫苗株相比,存在7个突变位点。流感暴发疫情病毒分离率42.6%,BV亚型3年分别占88.2%、61.9%、87.5%。结论郴州市流感的防控重点是B型流感病毒的流行,重点人群是0~14岁儿童,特别是中小学生;加强病毒变异监测,及时掌握变异动态。
Objective To understand the endemic situation and pathogenic characters of influenza in Chenzhou and to provide scientific evidence for formulating preventive and control strategies.Methods The basic data of influenza like illness（ILI）cases were collected from sentinel hospitals of Chenzhou in 2010-2012.Throat swab specimens were collected for virus isolation with MDCK cells,and identification by hemagglutination（HA） test and hemagglutination inhibition（HI） test.Results A total of 18,265 ILI cases were surveyed.The cases younger than 14 years old accounted for 71.7%,and the sex ratio was 1.54：1.3,859 throat swab specimens were collected,from which 242 virus strains were isolated and identified to be influenza viruses,with a virus isolation rate of 6.3%.The virus isolation rates from 2010 to 2012 were 4.8%,7.4% and 7.8%,respectively.Four subtypes,BV,BY,H3 and A-H1N1,were isolated,and BV was the main subtype through the 3 years.Compared with the vaccine strains,2 BY virus strains had 7 mutational sites.The virus isolation rate for influenza outbreaks was 42.6%,and the ratios of BV subtype in the 3 years were 88.2%,61.9% and 87.5%,respectively.Conclusions The prevention and control emphasis of influenza in Chenzhou is the epidemic of B influenza virus,and the key population is children younger than 14 years,especially primary and secondary school students.The virus variation surveillance should be enhanced to master the variation trend in time.
出处
《实用预防医学》
CAS
2013年第7期795-797,共3页
Practical Preventive Medicine
关键词
流感监测
流感病毒
病毒分离
Influenza surveillance
Influenza virus
Virus isolation
