摘要
目的研究骨髓间充质干细胞（MSC）诱导调节性B淋巴细胞（Breg细胞）的免疫负调节作用。方法取DA大鼠MSC和Lewis大鼠B淋巴细胞共培养后，酶联免疫吸附试验（EUSA）检测MSC和B淋巴细胞混合培养组上清液中白细胞介素10（IL-10）的水平和分泌因子的变化；采用流式细胞技术分析B淋巴细胞增殖、凋亡、表型的变化和对抗原特异性T淋巴细胞增殖的影响，采用激光共聚焦显微镜观察Breg细胞对免疫复合物吞噬能力的变化。结果MS（2成功诱导产生高分泌IL-10的Breg细胞产生，表型为CDl9＋CDld＋CD5＋，MSC显著抑制B淋巴细胞的增殖，Breg细胞能抑制抗原特异性T淋巴细胞的增殖，并增加对免疫复合物的吞噬能力。结论MS（2诱导的Breg细胞具有明显的免疫负调节作用。
Objective To observe the immunoregulatory roles of MSCs in inducing proliferation of B regulatory cells （Bregs）. Method DA Rat MSCs were co-cultured with Wistar rat B ceils. The levels of IL-10 and secrete proinflammatory cytokines in the supematants were determined by using ELISA. The proliferation and apoptosis of B cells after interaction with MSCs were analyzed by using FACS. In order to investigate whether Bregs are involved in the phagocytosis of immune complex （IC）, B ceils and Bregs were incubated with FITC-OVA IC. The fluorescence intensity was detected by using immunofluorescence microscopy. Results MSCs induced B cells to differentiate into Bregs with high CD19, CDld and CD5 expression and high IL-10 level. The proliferation of B cells was significantly suppressed after interaction with MSCs. Bregs had enhanced phagocytic capacity as compared with that of B cells. Bregs significantly inhibited maDC-induced CD4＋ T cell proliferation compared to B cells, without depressing IL-2 or IFN-7 secretion. Conclusion MSCs induce freshly isolated B cells to differentiate into Bregs characterized by the ability to preferentially produce IL-10.
出处
《中华器官移植杂志》
CAS
CSCD
北大核心
2013年第7期428-431,共4页
Chinese Journal of Organ Transplantation
基金
国家自然科学基金项目资助，江苏省333人才项目
关键词
大鼠
间质干细胞
B淋巴细胞
免疫耐受
Rats
Mesenchymal stem cells
B-Lymphocytes
Immune tolerance
