摘要
利用抑制消减杂交法从藜科盐穗木属盐生植物盐穗木中分离得到了一个盐胁迫响应的表达序列标签(EST)片段,结合SMARTTMRACE技术获得了盐穗木GRAS转录因子基因的cDNA。序列分析表明,该基因全长2 090bp,含有1 635bp的阅读框,294bp的5′-UTR和161bp的3′-UTR,编码544个氨基酸,分子质量为61.503kD,理论等电点为6.1。系统进化树和Blast同源序列比对分析结果显示,该基因编码的蛋白具有GRAS家族特有的C端保守结构域,并与葡萄GRAS家族蛋白VvSCL13聚集在一起,故将该基因命名为HcSCL13(GenBank登录号KC68640)。实时荧光定量qRT-PCR分析表明,HcSCL13基因在盐胁迫后表达呈明显上调,初步推测Hc-SCL13基因可能与盐穗木的耐盐性相关。
An cDNA fragment was isolated from Halostachys caspica by suppression subtractive hybridization and its full-length cDNA with 2 090bp was cloned by SMARTTMRACE,which consisted of a 1 635bp open reading frame encoding 544 amimo acids with molecular weight of 61.503kD and an isoelectric point of 6.1,a 294bp 5′-UTR and 161bp 3′-UTR.The deduced amino acid sequence got together with that of GRAS family protein VvSCL13from Vitis vinifera and had a C-terminal conserved domain,so it was named HcSCL13 gene（GenBank accession number KC68640）.Real-time PCR method was performed to investigate the expression profile of HcSCL13 gene under salt stress.HcSCL13showed up-regulated expression patterns under salt treatment.Based on our results,we concluded that HcSCL13 gene might be involved in salt response and one of important components for the salt tolerant pathway in H.caspica.
出处
《西北植物学报》
CAS
CSCD
北大核心
2013年第6期1091-1097,共7页
Acta Botanica Boreali-Occidentalia Sinica
基金
973计划前期研究专项(2012CB722204)
新疆维吾尔自治区高校科研计划科学研究重点项目(XJEDU2012102)
新疆大学博士启动基金(XJEDU2009S04)
新疆生物资源基因工程重点实验室开放课题(XJDX0201-2013-02)
新疆大学大学生重点实训项目资助(XJU-SRT-Z11016)
