摘要
探讨苦马豆素(Swainsonine,SW)对小鼠脑组织抗氧化功能的影响,进一步揭示SW的毒性作用机理。将64只昆明种小鼠随机分为对照组、试验Ⅰ组、试验Ⅱ组和试验Ⅲ组,将小花棘豆全草粉碎后,按Ⅰ组添加15%(SW含量30mg/kg)、Ⅱ组添加30%(SW含量60mg/kg)、Ⅲ组添加45%(SW含量90mg/kg)的比例制作混合饲料,饲喂至典型中毒症状出现。攻毒后第14、28、42、63天每次每组随机采集4只小鼠的脑组织,检测超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)、过氧化氢酶(CAT)、一氧化氮合酶(NOS)活性及丙二醛(MDA)、游离脂肪酸(NEFA)、羟自由基(·OH)、脂褐素(LPO)、一氧化氮(NO)和游离谷氨酸(Glu)含量的变化。结果表明,与正常对照相比,试验组小鼠脑组织SOD、GSH-Px、CAT等抗氧化物酶的活性极显著下降(P<0.01),而MDA、NEFA、·OH、LPO、NO和Glu含量极显著上升(P<0.01)。SW对小鼠脑组织抗氧化功能有显著影响,且具有一定的时间效应和剂量效应关系,长期低剂量摄入SW可导致小鼠不同程度的脑损伤。
This study was to investigate the effects of swainsonine（SW） on the antioxydic function of brain in mice, and reveal the toxicity mechanism of SW in future. Sixty four Kunming mice were divided into 4 groups （the control group and experimental group Ⅰ , Ⅱ , Ⅲ ） , the dried plant of Oxytro pis glabra DC was comminuted, and different amounts of the grass powder（15Y00, 30% and 45G the corresponding SW content was 30 mg/kg, 60 mg/kg, 90 mg/kg） were mixed with the feeds in the three experimental groups. The mice consumed the forages freely until typical symptoms were ob- served. Four mice were selected randomly from each group on the 14th, 28th, 42nd and 63rd day re- spectively, the brain was collected to measure the activities of superoxide dismutase （SOD）, glutathi one peroxidase （GSH Px）, eatalase（CAT） and nitric oxide synthase（NOS）, the contents of maleie di- aldehyde （MDA）, renal free fatty acids（NEFA）, hydroxyl radical（ · OH）, lipofuscim（LPO）, nitric oxide（NO）and glutamic acid（Olu）. The results demonstrated that the activity of SOD, GSH-Px and CAT in experimental groups were significantly lower than normal control group（P〈0.01）, and thecontents of MDA, NEFA, · OH, LPO, NO and Glu in experimental groups were significantly higher than normal control group（P〈0.01）. The results showed that SW has remarkable effects on the an- tioxydic function of brain in mice, with time-effect and dose-effect. It is concluded that long-time con- suming swainsonine at a low-dosage will cause brain damage in mice.
出处
《西北农业学报》
CAS
CSCD
北大核心
2013年第7期12-18,共7页
Acta Agriculturae Boreali-occidentalis Sinica
基金
国家自然科学基金(31160497)
新疆生产建设兵团博士基金专项(2008JC07)
新疆生产建设兵团塔里木畜牧科技重点实验室开放课题(HS201207)
关键词
苦马豆素
自由基
小鼠
脑
Swainsonine
Free radical
Mice
Brain
