摘要
以4份不同发芽水平的老芒麦(Elymus sibiricus Linn.)种子作为试验材料,研究比较老化温度(39、41、43℃和45℃)和老化时间(1、2、3、4、5、6、7 d)处理对种子发芽率的影响,筛选确定老芒麦种子进行人工加速老化测定的适宜条件,并建立标准化的测定程序。结果表明:随着老化温度升高,Lot1、Lot2和Lot3样品种子发芽率均下降,且各处理间均差异显著(p<0.05),只有Lot 3在45℃条件下老化后发芽率下降的比例最低;Lot 4样品种子老化后发芽率较低,且45℃处理与其他各处理间均差异显著(p<0.05)。随着老化时间延长,Lot 1和Lot 2老芒麦种子老化发芽率不断降低,老化1、2 d处理与其他各处理发芽率间差异显著(p<0.05);Lot 3和Lot 4老芒麦种子老化至第4天后发芽率出现峰值。老化温度与老化时间互作效应与种子发芽率和休眠程度有关,且对老芒麦种子老化发芽率具有极显著(p<0.01)的影响。老芒麦种子人工加速老化处理的适宜条件为温度45℃老化2 d。
Four samples of Siberian wildrye（Elymus sibiricus Linn.） seeds with different germination level were used in the experiment and conducted to analyze the effect of the ageing temperature（39,41,43 and 45 ℃） and ageing time（1,2,3,4,5,6 and 7 d） on the germination percentage,and to find out the suitable condition and procedure for the accelerated ageing test of Siberian wildrye seeds.The result indicated that the seed germination of Lot 1,Lot 2 and Lot 3 decreased,and the germination decrease had significant difference（p 0.05） at 39 ℃,41 ℃,43 ℃ and 45 ℃,but only at 45 ℃ the aging germination percentage of Lot 3 fell lowest.The aging germination percentage of Lot 4 seed sample was low,and there was significant difference（p 0.05） between 45 ℃ and the other treatments.The seed germination of Lot 1 and Lot 2 gradually decreased with the treatments of the ageing time from 1 d to 7 d,and the germination after ageing 1 d and 2 d were significant different（p 0.05） with others.Germination of Lot 3 and Lot 4 had peak value after ageing 4 d,and was significantly（p 0.05） different from ageing 2,3,5,6 and 7 d.The interaction between the ageing temperature and ageing time was connected with the standard germination percentage and dormancy of seed,and significantly（p 0.01） related with the germination rate after ageing of Siberian wildrye seeds.The result indicated that ageing temperature 45 ℃ was optimum temperature and ageing time 2 d was optimum time for accelerated ageing test of Siberian wildrye seeds.
出处
《种子》
CSCD
北大核心
2013年第7期1-6,共6页
Seed
基金
中国农业大学青年教师科研项目
现代牧草产业技术体系(编号:CARS-35)
博士点基金项目(编号:20110008110003)
十二.五科技支撑课题"优质多抗牧草新品种选育与良种繁育关键技术研究与示范"(编号:2011BAD17B01-02)
草业科学北京市重点实验室共建项目资助
关键词
老芒麦种子
活力测定
人工加速老化
休眠
siberian wildrye seed
seed vigor testing
artificial accelerated aging
dormancy
