摘要
目的:考察载阿霉素金磁复合微粒联合外磁场在荷瘤小鼠体内靶向分布特性及其抗肿瘤效果。方法:建立小鼠肝细胞癌动物模型,取50只,随机分为5组,采用尾缘静脉注射法,各组给予不同剂型药物,磁阿霉素组加外磁场固定2 h。每3天1次,分别于给药后第6天和第9天测算肿瘤抑制率,评价其靶向抗肿瘤效果;另取30只,随机分为3组,经尾缘静脉给予不同剂型药物,磁阿霉素组外磁场固定1 h,然后处死小鼠,心脏取血并收集心、肝、肾、肿瘤组织,应用高相液相色谱法测定各组织中阿霉素浓度,考察其在小鼠体内靶向分布特性。结果:靶向抑瘤实验显示,在各种不同剂型给药中,磁阿霉素联合磁场组较其他给药组均有明显的抑瘤效果(P=0.000),并在持续给药的情况下,对肿瘤抑制效果不断增强,而磁阿霉素组在无外加磁场的环境下,其抑瘤效果与单纯阿霉素给药效果无差异,单纯磁微粒组无抑瘤作用。靶向分布特性实验结果表明,肿瘤靶区应用磁场固定1 h后,肿瘤组织中药物浓度较非磁区组织中的药物浓度明显增高,对照组在没有磁场存在的情况下,肿瘤组织中药物浓度与非磁区组织的药物浓度基本相同。结论:载阿霉素金磁复合微粒联合外磁场对小鼠肝细胞癌具有良好的靶向定位作用和明显的肿瘤抑制效果,有望成为新型药物用于临床肿瘤的靶向治疗。
Objectives To study the in-vivo targeting distribution and anti-tumor effect of doxorubicin- loaded magnetic composite particles combined with an external magnetic field. Methods 50 mice with hepatocellular carcinoma were randomly divided into 5 groups, and received drugs with different dosage forms by trail edge intravenous injection respectively. Mice in doxorubiein-loaded magnetic composite particle group were given an external magnetic field for 2 h. On day 6 and 9 after administration, the tumor inhibition ratio was detected and calculated, the targeting anti-tumor effect was evaluated. Another 30 mice were randomly divided into 3 groups and received drugs with different dosage forms by trail edge intravenous injection respectively; and mice in doxorubicin-loaded magnetic composite partiele group were given an external magnetic field for l h, and then all mice were sacrificed; blood in hearts was sampled; liver, kidney, heart and the solid tumor were collected. Finally the targeting distribution was studied by determining the concentration of doxorubicin in each tissue through HPLC. Resdts The study of targeting tumor inhibition indicated that, for all dosage forms, combination of doxorubicin- loaded magnetic composite particles with an external magnetic field was statistically different from other groups in tumor inhibition （P = 0.000）, which was increased with continuous administration. There was no significant difference between doxorubicin-loaded magnetic composite particles group and doxorubicin group. The magnetic composite particle group didn＇t show any tumor inhibition. In the targeting distribution study, the drug coneentration in tumor received 1 h of magnetic field was significantly higher than that in tissues did not received magnetic field. The drug concentration in tumor was the same as that in other tissues if no external magnetic field was given. Conclusion Combination of doxorubicin-loaded magnetic composite particles with extemal magnetic had satisfied targeting location and significant tumor inhibition on mice with hepatocellular carcinoma, and could be a new targeted therapy for tumor.
出处
《实用医学杂志》
CAS
北大核心
2013年第15期2465-2468,共4页
The Journal of Practical Medicine
基金
2012年地方高校国家大学生创新创业计划项目(编号:201211258021)
关键词
肝肿瘤
阿霉素
金磁复合微粒
磁靶向性
抗肿瘤效果
Liver neoplasms
Doxorubicin
Magnetic composite particles
Magnetic targeting
Anti-tumor effect
