摘要
本试验将原代培养的籽鹅卵泡颗粒细胞转染ENO1腺病毒过表达载体,应用实时荧光定量PCR方法检测ENO1过表达对颗粒细胞Bcl-2mRNA表达量的影响。结果发现ENO1过表达使卵泡颗粒细胞Bcl-2mRNA表达量极显著增加(P<0.01)。提示ENO1可能通过使Bcl-2基因表达量增加,从而抑制籽鹅卵泡颗粒细胞凋亡,促进卵泡的发育。
The paper was to study the effects of ENO1 overexpression with adenovirus vector on Bel-2 in the granulose cell of Zi goose. Adenovirus vector with ENO1 were transfected to Zi goose primary cultured granulose cells, and Bcl-2 mRNA of granulose cells were detected by the method of Real-time fluorescence quantitative PCR. The results showed that ENO1 over expression made the Bcl-2 mRNA expression in the granulose cells significantly increase （P〈0.01）. ENO1 overexpression made the primary culture follicle granulose cells in vitro improve Bcl-2 mRNA expression, which suggested that ENO1 might inhibit the apoptpsis of Zi ~oose ~zranulose cell.
出处
《中国畜牧兽医》
CAS
北大核心
2013年第7期10-13,共4页
China Animal Husbandry & Veterinary Medicine
基金
黑龙江省自然科学基金重点项目(ZD20116)
