摘要
以构建水稻osvdac3基因超表达载体并获得超表达的转基因水稻植株为目标,通过PCR扩增将水稻osvdac3基因的全长片段克隆到植物表达载体,构建CaMV35S∷osvdac3∷rfp植物超表达载体。并以水稻YTB品种作为其遗传转化的受体,通过农杆菌介导的愈伤组织侵染法进行水稻遗传转化。结果表明,利用该方法成功构建了水稻osvdac3基因超表达载体,在此基础上获得了多个osvdac3基因超表达的水稻植株,并使用RT-PCR技术分析了阳性植株中osvdac3基因的表达水平。该研究结果为进一步研究水稻osvdac3基因蛋白的功能奠定了基础。
The goal of the research was to construct the over-expression vector of osvdac3 and to obtain the transgenic plants. Cloning the osvdac3 full-length gene into the plant expression vector by PCR,the over-expression vector was constructed with CaMV35S ：： osvdac3 ：： rfp. Through Agrobacterium-mediated approach, the recombinant plasmid was transformed into the rice YTB＇s callus. The results showed that the over-expression vector of CaMV35S ：： osvdac3 ：： rfp was constructed successfully and some transgenic plants were obtained. Meanwhile, the expression level of osvdac3 in the transgenic plants was confirmed by RT-PCR. The results laid the basic foundation for further function research of osvdac3.
出处
《广东农业科学》
CAS
CSCD
北大核心
2013年第14期13-15,共3页
Guangdong Agricultural Sciences
基金
国家自然科学基金面上项目(31170226)
湖北省自然科学基金(2012FFB07407)
