摘要
为了明确江西省辣椒疫霉对烯酰吗啉的抗药性风险,采用灌根法鉴定了108个江西辣椒疫霉菌株的生理小种,并采用菌丝生长速率法测定了江西省辣椒疫霉对烯酰吗啉的敏感性。结果表明,菌株LP20、LP38和LP48属于生理小种3,占测定菌株总数的2.8%,其余菌株属于生理小种2,占测定菌株总数的97.2%,为优势生理小种,且未发现生理小种1。烯酰吗啉抑制辣椒疫霉菌丝生长的EC50值范围为0.1149-0.2868μg/mL,最不敏感菌株的EC加值为最敏感菌株的2.49倍。不同地区的菌株对烯酰吗啉的敏感性差异不大,崇义的菌株平均EC50值最低,为0.1367μg/mL,分布范围为0.1213—0.1462μg/mL;吉水的菌株平均EC50值最高,为0.2185μg/mL,分布范围为0.1805~0.2835μg/mL。
In order to provide a theoretical basis for pepper breeding and gain a clear idea of the resist- ance risk of Phytophthora capsici to dimethomorph in Jiangxi Province, the physiological races were iden- tified with root-irrigating method and the sensitivity of P. capsici to dimethomorph was determined with mycelial growth inhibition method. The results showed that, among the 108 P. capsici strains in Jiangxi, no strain belonged to Race 1, three strains belonged to Race 3 and 105 strains belonged to Race 2. The ratio of Race 2 was 97.2%, which indicated that Race 2 was the superior race in Jiangxi Province. The ECso values of 108 P. capsici strains to dimethomorph ranged from 0.1149 μg/mL to 0. 2868 μg/mL. The EC50 value of the most insensitive strain was 2.49 folds of that of the most sensitive strain, with a mean of 0. 1845 μg/mL. The EC50 values of the strains collected from Chongyi ranged from 0. 1213 μg/mL to 0. 1462 μg/mL with a minimum mean of 0. 1367 μg/mL, and the EC50 values of the strains collected from Jishui ranged from 0. 1805μg/mL to 0. 2835 μg/mL with a maximum mean of 0.2185 μg/mL. The above data showed that there was no significant difference in sensitivity of strains to dimethomorph among differ- ent areas.
出处
《植物保护学报》
CAS
CSCD
北大核心
2013年第4期374-378,共5页
Journal of Plant Protection
基金
公益性行业(农业)科研专项(201003004)
江西省科技支撑重点项目(2010BNA04900)
江西省农业科学院科技创新及成果转化基金项目
关键词
辣椒疫霉
生理小种
烯酰吗啉
敏感性
Phytophthora capsici
physiological race
dimethomorph
sensitivity