摘要
目的评价芯片传感-微分电位溶出法在临床血铅检测的准确度和重复性,并与氢化物发生原子荧光法比较,为临床选择检测方法提供参考依据。方法分别运用芯片传感-微分电位溶出法和氢化物发生原子荧光法同时检测血液样本和标准物质(为2011年全国血铅检测室间质评样本),评价芯片传感-微分电位溶出法的正确度、重复性、回收率、可报告范围和符合率。结果以靶值±10%作为允许误差,6种标准物质的芯片传感-微分电位溶出法测定结果与靶值比较,均在允许误差范围之内,正确度良好;低、中、高3个水平样本的相对标准偏差(RSD)分别为6.98%、4.09%、2.78%;平均回收率为98.1%,系统比例误差为1.9%;与氢化物发生原子荧光法比较,差异无统计学意义(P>0.05),线性回归分析相关系数(r2)=0.964,2种方法测定结果的符合度为96.4%。结论芯片传感-微分电位溶出法准确度高、重复性好、精密度佳,适用于临床血铅检测。
Objective To evaluate the accuracy and repeatability of clinical blood lead detection by sensor chip- differential potentiometric stripping analysis and compare with hydride generation atomic fluorescence spectrometry, and to provide the reference for clinical application. Methods The 2 methods were used to analyze the blood samples and the certified reference materials （ national blood lead detection external quality assessment samples in 2011 ）. The accuracy, repeatability, recovery rate, acceptable range and coincidence rate of sensor chip-differential potentiometric stripping analysis were evaluated. Results All of the results of 6 certified reference materials were in the allowable error range（target value ＋ 10% ）, and the accuracy was good. The relative standard deviations （RSD） of the low, medium and high blood lead level blood samples were 6.98% ,4.09% and 2.78% , respectively. The average recovery rate was 98.1% , and the system ratio error was 1.9%. Compared the results of sensor chip-differential potentiometric stripping analysis and hydride generation atomic fluorescence spectrometry, the results had no statistical significance （ P 〉 0.05 ） , and the correlation coefficient of linear regression analysis （ rE ） was O. 964. The coincidence rate of the 2 methods was 96.4%. Conclusions sensor chip-differential potentiometric stripping analysis has good accuracy, repeatability and precision, and is suitable for the clinical application of detecting blood lead.
出处
《检验医学》
CAS
2013年第8期704-706,共3页
Laboratory Medicine
关键词
血铅
芯片传感器
微分电位溶出法
氢化物发生原子荧光法
Blood lead
Sensor chip
Differential potentiometric stripping analysis
Hydride generation atomicfluorescence spectrometry
