摘要
目的:建立一测多评法(QAMS)同步测定复方土荆皮酊中土荆皮乙酸-O-β-D-葡萄糖苷(PABG)、土荆皮丙酸(PAC)和土荆皮乙酸(PAB)3种二萜类成分的含量。方法:以土荆皮乙酸为内参物,建立其与土荆皮乙酸-O-β-D-葡萄糖苷和土荆皮丙酸的相对校正因子(RCF)(分别是0.681 4和1.225 3),利用该校正因子计算其他2种二萜类成分的含量,实现一测多评;同时采用外标法测定17批次复方土荆皮酊中3种二萜类成分的含量,比较计算值与实测值间的差异,验证一测多评法的准确性和可行性。结果:可以采用一测多评法对17批次复方土荆皮酊中的3种二萜类成分进行测定,其计算值与实测值间无显著差异,实验所得的校正因子可信。结论:建立的一测多评法同步测定复方土荆皮酊中3种二萜类成分的含量是可行的、准确的,可用于复方土荆皮酊的质量评价。
Objective：To establish a method of multi-assay by single-marker（QAMS） for simultaneous determination of pseudolaric acid B-O-β-D-glucoside（PABG）,pseudolaric acid C（PAC） and pseudolaric acid B（PAB） in Fufang Tujingpiding.Method：Using pseudolaric acid B as the internal reference substance,the relative correction factors（RCF） of the other two diterpenoids were 0.681 4 and 1.225 3 respectively.The other two diterpenoids were simultaneously calculated according to their RCFs.The content of these two diterpenoids in 17 samples was also determined by external standard method,comparing two methods to validate the accuracy and feasibility of the new method.Result：Three diterpenoids in 17 batches of Fufang Tujingpiding were determined by the new method,and there were no significant differences between the calculated values and the measured values.Conclusion：The new method is feasible and accurate to evaluate the content of three diterpenoids in Fufang Tujingpiding,which can be used as the quality control of Fufang Tujingpiding.
出处
《中国实验方剂学杂志》
CAS
北大核心
2013年第17期77-80,共4页
Chinese Journal of Experimental Traditional Medical Formulae
基金
军队十二五规划项目
关键词
一测多评
相对校正因子
高效液相色谱
复方土荆皮酊
二萜类成分
quantitative analysis of multi-components by single marker（QAMS）
relative correction factor
HPLC
Fufang Tujingpiding
diterpenoid components
