摘要
目的研究兔死后角膜厚度变化规律与死亡时间（postmortem interval,PMI）的关系。方法用空气栓塞法建立兔死亡模型。在兔死后0－72h,每隔6h进行角膜取材,切片后常规HE染色,用光学显微镜摄取全角膜图像,结合Motic Images Plus 2.0图像分析软件观察角膜上皮层厚度（x1）、角膜基质层厚度（x2）、全角膜厚度（x3）3项指标的变化值,并作统计学分析,建立与PMI（y）的回归方程。结果在兔死后72 h内,角膜基质层厚度和全角膜厚度均在死后12h开始增加,并在死后54h达到高峰,两者与PMI正相关,回归方程分别为y=-0.070 2 x22＋11.398 x2＋1 634（R2=0.712 2,P〈0.05）,y=-0.074 9 x32＋12.036 x3＋1 819.4（R2=0.675 0,P〈0.05）。结论角膜基质层厚度和全角膜厚度与PMI之间呈较好的线性关系,且角膜基质层厚度优于全角膜厚度,可作为推断死亡时间的指标。
Objective To explore the relationship between corneal thickness and postmortem interval（PMI） in rabbit.Methods The rabbit model was established by air embolism.The rabbit cornea was sampled at 6-hour-interval from 0 to 72 h postmortem.After routine HE staining,the whole cornea image was collected by the optical microscope.Three markers were observed including corneal epithelial thickness（x 1）,corneal stromal thickness（x 2） and whole corneal thickness（x 3） using Motic Images Plus 2.0 image analysis software and the data were statistically analyzed to establish the regression function with PMI（y）.Results Within 72 h postmortem,rabbit corneal stromal thickness and whole corneal thickness increased at 12 h postmortem and reached the peak at 54 h postmortem.The two markers showed positive correlation with PMI.The regression functions of the two markers were y=-0.070 2 x 2 2 ＋11.398 x 2 ＋1 634（R 2 =0.712 2,P0.05） and y=-0.074 9 x 3 2 ＋12.036 x 3 ＋1 819.4（R 2 =0.675 0,P0.05）,respectively.Conclusion The two markers of corneal stromal thickness and the whole corneal thickness showed the strong linear correlation with PMI.The correlation of the corneal stromal thickness is better than the whole corneal thickness.The two markers can be used to estimate PMI.
出处
《法医学杂志》
CAS
CSCD
2013年第4期241-243,共3页
Journal of Forensic Medicine
关键词
法医病理学
角膜
死亡时间
兔
forensic pathology
cornea
postmortem interval
rabbits
