摘要
通过化学和形态分析手段,研究了碱性木聚糖复合酶处理中酶用量、温度、pH值对桑芯主要化学组分、得率和组织形态的影响。研究表明:自制的酶是耐温耐碱、含有少量果胶酶和纤维素酶的木聚糖复合酶,对温度和pH值的适应范围较广;在优化的温度和pH值条件下,随着酶用量的增加,桑芯中木质素和综纤维素都呈现下降的趋势,但得率均高于95%;优化的酶处理条件是:酶用量2.0 U/g、温度50℃、pH值8.0;酶处理前后组织、细胞形态发生了明显变化,经过酶处理后,细胞得到较充分润胀,纤维表面变粗糙,增加了组织的多孔性和细胞的比表面积,将提高桑枝的物理、化学及生物转化过程化学药品渗透和反应性,有利于提高生物炼制的效率。
The effects of xylanase treatment with variable enzyme dosage under different pH and temperature on the changes of chemical composition, pulp yield, and fiber morphologies were investigated in this study. The results indicated the homemade xy- lanase was a thermostable and alkaline-tolerant complex enzyme with minor parts of pectinase and cellulase, and could be applied in a wide range of pH and temperature. The optimized enzyme treatment conditions were 2.0 U/g of enzyme dosage, 50~C of re- action temperature and pH 8.0, respectively. The holocellulose, hemieelluloses and lignin contents declined with the enzyme dosage increasing. The pulp yield exceeded 95% under this optimized reaction temperature and pH,. The swollen fiber walls and coarse fiber surfaces were observed after the enzyme treatment. The fiber porosity and specific surface area increased as well. This could improve the penetration and reactivity of the chemicals in aspects of physical, chemical and biological acting proees, ses, and was beneficial to improve the bio-refinery efficiency.
出处
《林产化学与工业》
EI
CAS
CSCD
北大核心
2013年第4期9-14,共6页
Chemistry and Industry of Forest Products
基金
江苏省科技创新与成果转化专项(BE2010689
BE2010069)
关键词
木聚糖复合酶
桑芯
化学成分
纤维形态
complex xylanase
mulberry core
chemical composition
fiber morphology
