摘要
生物素与亲和素具有很强的亲和力，本文利用这一特性建立了一种直接竞争抑制的酶联免疫法（ＥＬＩＳＡ），用于检测微生物发酵液中生物素含量．当标准品生物素含量在０．５～２０μｍｏｌ／Ｌ范围内，它与抑制率（Ｉ）负对数呈线性关系，因此发酵液样品中生物素含量可从标准曲线上查得。该法简便、准确，而且不需对样品作任何预处理。
Based on the strong affinity between biotin and avidin, direct compeitive inhibitory ELISA, which was applied to the determination of biotin in fermentation liquid was proposed. A linear response between the concentration of biotin and- lgI was obtained in the range of 0.5~ 20μmol / L. So the level of biotin in fermentation liquid could be calculated from the standard curve. The method was simple, rapid and accurate. Any pretreatment was not necessary for samples.
出处
《微生物学通报》
CAS
CSCD
北大核心
2000年第1期47-50,共4页
Microbiology China
基金
浙江省分析测试基金
