摘要
目的:探讨学龄前原发性肾病综合征(PNS)患儿淋巴细胞亚群的变化。方法:选择学龄前PNS患儿86例,体检正常儿童20例,采集清晨空腹静脉血2mL,应用流式细胞术检测各淋巴细胞亚群的比例,分析学龄前PNS患儿淋巴细胞亚群比例的变化与激素敏感和复发的关系。结果:学龄前PNS患儿CD3+、CD4+、CD8+T淋巴细胞比例,CD4+/CD8+,NK细胞比例与正常儿童相比显著降低(t=6.529、7.838、3.480、2.777和5.351,P<0.05);激素耐药组CD8+T淋巴细胞比例低于激素敏感组(t=2.124,P=0.036),CD4+/CD8+、NK细胞比例高于激素敏感组(t=2.608和2.727,P<0.05);复发组及频复发组CD8+T淋巴细胞比例高于未复发组,CD4+/CD8+、NK细胞比例低于未复发组(P<0.05);频复发组NK细胞比例低于复发组(P<0.05)。结论:学龄前PNS患儿存在T淋巴细胞亚群比例失衡,其激素治疗的敏感性和复发亦与该失衡有关。
Aim： To explore the varieties of lymphocyte subsets in pre-school children with primary nephrotic snydrome（PNS） ,and the revelance to steroid sensitiviness and relapse.Methods： The varieties of lymphocyte subsets in 86 pre-school children with PNS（PNS group） and 20 healthy children（control group） were detected by flow cytometry.Results： The CD3＋ T lymphocytes,CD4＋ T lymphocytes,CD8＋ T lymphocytes,CD4＋/CD8＋ ,NK cells of pre-school children with PNS were obviously less than those of normal children（t=6.529,7.838,3.480,2.777,and 5.351,P0.05） .Compared with PNS children with steroid sensitive,the steroid resistant children＇s CD8＋ T lymphocytes decreased （t=2.124,P=0.036） ,and the CD4＋/CD8＋ ,NK cells increased（t=2.608 and 2.727,P0.05） .Compared with PNS children without relapse,the relapse group and frequent relapse group＇s CD8＋ T lymphocytes increased,and the CD4＋/CD8＋ , NK cells decreased（P0.05） .The NK cells of frequent relapse group was less than those of relapse group（P0.05） .Conclusion： Pre-school children with PNS exist the imbalance of T lymphocyte subsets percentage,which may be more prone to have steroid resistant and relapse.
出处
《郑州大学学报（医学版）》
CAS
北大核心
2013年第4期547-550,共4页
Journal of Zhengzhou University(Medical Sciences)
