摘要
目的观察急性低压缺氧对大鼠肾脏的损害。方法将 36只雄性Wistar大鼠随机平均分为A、B、C三组。A组为对照组 ,将B、C两组置低压舱内上升至 50 0 0m ,停留 30min ,予以缺氧。下降后B组2 0min后 ,C组 2 4h后取静脉血及肾组织 ,用全自动生化分析仪测定血清尿素氮、肌酐、碱性磷酸酶、氯离子 ,并用放免法测定肾组织内皮素。切取肾皮质做光镜及电镜下的病理检查。结果实验组血清尿素氮和肌酐明显增多 (P <0 .0 1 ) ,氯离子和碱性磷酸酶也增多 (P <0 .0 5) ,而肾内皮素明显减少 (P <0 .0 1 ) ,2 4h内各项指标向正常恢复 ,只有氯离子继续增大。光镜下可见肾血管的舒张 ,线粒体、上皮细胞有病理变化。结论急性低压缺氧对肾功能和肾脏结构均有损害。
Objective To observe renal damage due to acute hypobaric hypoxia. Method Thirty six male wistar rats were randomly divided into three groups A, B and C(n=12 for each).Group A served as control,while groups B and C were exposed to 5000 m altitude for 30 min.Sample of serum and renal tissues were taken from group B rats 20 min and from group C rats 24 h after the exposure respectively. Result Serum ureanitrogen (BUN) and creatinine (Crea) increased significantly in group B and C rats after exposure to hypoxia(P<0.01).Cl - and alkaline phosphatase (AKP) in serum also increased (P<0.05),but renal endothelin(ET) decreased significantly (P<0.01).All changes in group C rats tended to recover to normal level, except Cl - which continued to increase. Dilation of renal vessels was found under optic microscope,and pathological changes were found in mitochondra and epithelial cells. Conclusion Acute hypobaric hypoxia might be harmful to renal function and structure.
出处
《航天医学与医学工程》
CAS
CSCD
2000年第3期215-217,共3页
Space Medicine & Medical Engineering
关键词
缺氧
失重模拟
肾功能
组织损伤
大鼠
hypoxia
weightlessness simulation
renal function
histologic lesions
rats
