摘要
目的 探讨肿瘤坏死因子 -α(TNF-α)对周围神经损伤后的脊髓前角运动神经元胞体的保护作用。方法 Wistar大鼠 2 0只 ,随机分为实验组与对照组 ,每组 10只。切断右侧坐骨神经 ,近端套入硅胶管内 ,管内分别注入生理盐水 (NS)、TNF-α (30 U /ml)各 16μl。术后 2周 ,取腰 4、5脊髓 ,行乙酰胆碱酯酶 (ACh E)、一氧化氮合酶(NOS)染色。应用计算机图像分析系统计算出脊髓前角运动神经元 ACh E、NOS的阳性数目。结果 两组间ACh E、NOS的活性有显著改变 ,实验组 ACh E阳性数目为 8.6 5± 1.98,NOS的阳性数目为 5 .92± 1.36 ;对照组ACh E阳性数目为 6 .37± 1.42 ,NOS阳性数目为 8.6 7± 1.45 ,经统计学处理有显著性差异 (P<0 .0 1)。结论 TNF-α对周围神经损伤后脊髓前角运动神经元有保护作用。
Objective To investigate the protective effect of tumor necrosis factor-α(TNF-α) on spinal motor neurons after peripheral nerve injury. Methods Twenty Wistar rats were divided into two groups, the right sciatic nerves of 20 Wistar rats were transected, the proximal stumps were inserted into a single blind silicone tube. 16μl of normal saline(NS) and TNF-α(30 U/ml) were injected into the silicone tubes. After 2 weeks,the 4th, 5th lumbar spinal cord were taken for examination. Enzyme histochemical technique and image analysis were used to show acetylcholinesterase(AChE) and nitric oxide synthase(NOS) activity of spinal motor neruons. Results The number of AChE and NOS staining neurons were 8.65±1.98 and 5.92±1.36 in the experimental group and 6.37±1.42 and 8.67±1.45 in the control group respectively, there were significant difference between the two groups(P<0.01). Conclusion It suggests that TNF-α has protective effect on motor neurons after peripheral nerve injury.
出处
《中国修复重建外科杂志》
CAS
CSCD
2000年第6期343-345,共3页
Chinese Journal of Reparative and Reconstructive Surgery
