摘要
目的 明确喉部良、恶性肿瘤与人类乳头状瘤病毒 (HPV)之间的关系。方法 采用型特异性聚合酶反应(PCR)技术 ,对 6 0例喉癌、36例喉乳头状瘤及 78例声带息肉的新鲜冰冻组织标本进行HPV DNA的检测。结果 HPV 16型阳性者在喉癌组为 2 0例 (33 .33% ) ,喉乳头状瘤组为 4例 (11.11% )及声带息肉组为 4例 (5 .13 % ) (P <0 .0 0 5 ) ;HPV 6 /11型阳性者在喉乳头状瘤组为 35例 (97.2 2 % ) ,喉癌组为 14例 (2 3.33% )及声带息肉组为 16例(2 0 .5 1% ) (P <0 .0 0 5 )。HPV16型感染与喉癌的生物学行为不相关 (P >0 .0 5 ) ;HPV 16型阳性者在吸烟患者中(4 0 % )明显高于非吸烟喉癌患者 (13.33% ) (P <0 .0 5 )。同时还发现 2例恶变的喉乳头状瘤患者均有吸烟史和检出HPV 16型。结论 HPV分型与喉部疾病的良、恶性有关 ;HPV 16型感染与吸烟是喉癌发病学中不容忽视的致癌因素 ;HPV分型的检测对判断喉乳头状瘤的转归有意义。
Objective To investigate the relationship between laryngeal malignant and benign tumor and human papilloma virus(HPV).Methods Freshly frozen tissue samples of 60 laryngeal and carcinoma,36 laryngeal papilloma and 78 laryngeal polyp were examined for the presence of HPV DNA using polymerase chain reaction(PCR).Results HPV genomes 16 was detected in 20 of 60(33.33%) laryngeal carcinoma,in 4 of 36( 11.11% ) laryngeal papilloma and in 4 of 78(5.13%) laryngeal polyp( P <0.005);HPV genomes 6/11 was detected in 35 of 36 (97.22%) laryngeal papilloma, 4 of 60 (23.33%) laryngeal carcinoma and 16 of 78(20.51%) laryngeal polyp( P <0.005).The infection of HPV 16 had no correlation with laryngeal carcinoma( P >0.05),It could be concluded that the positive rate of HPV genomes 16 in the smoking patients(18/45,40%) was obvious highly than that in nonsmoking(2/15,13.33%)( P <0.05),and that two malignant laryngeal carcinoma patients had smoking history and positive HPV genomes 16. Conclusion There is a certain relationship between HPV and characteristic of laryngeal tumor.HPV16 and smoking are two important factors which may be closely association with development of laryngeal carcinoma.The examination of the type of HPV plays an important role in the outcome of laryngeal papilloma.
出处
《哈尔滨医科大学学报》
CAS
2000年第5期319-322,共4页
Journal of Harbin Medical University
基金
黑龙江省科委攻关课题!(G97C18 3 18)
关键词
人乳头状瘤病毒
喉癌
喉乳头状瘤
声带息肉
human papilloma virus
polymerase chain reaction
laryngeal carcinoma
laryngeal papilloma
laryngeal polyp
