摘要
以不同比例大豆浓缩蛋白和花生麸为混合植物蛋白源替代不同水平鱼粉，并添加包膜赖氨酸和蛋氨酸，饲喂斑节对虾（Penaeus morlodoll）[（1．27±0．02）g]56d，研究不同鱼粉含量饲料对斑节对虾生长及非特异性免疫力的影响。结果表明：1）斑节对虾增重率（245．02％）、特定生长率（2．58％·d^-1）均在对照组获得最大值，显著高于除F1和F2组外的其他各组（P〈0．05）。当对虾摄食F1组饲料时饲料系数最小，显著小于F3～F5组（P〈0．05）。成活率在F4组最低，显著低于F0～F2组（P〈0．05），但与其他各组无显著性差异（P〉0．05）；2）混合植物蛋白替代不同水平鱼粉的饲料对虾体成分无显著性影响（P〉0．05）；3）肝脏中超氧化物歧化酶（SOD）活性在F2组最高，显著高于对照组（P〈0．05），与其他各组差异不显著（P〉0．05）。肌肉中S01）活性在F5组最高，显著高于其他各组（P〈0．05），酸性磷酸酶（ACP）活性在F1组最高，显著高于F2～F4组（P〈0．05），溶菌酶（LSZ）活性在F4和F5组最高，显著高于F1D～F3组（P〈0．05）。根据斑节对虾的增电率和特定生长率得出用混合植物蛋白并添加包膜氨基酸可替代饲料中20％的鱼粉，且随着饲料中植物蛋向含量的增加，对虾非特异性免疫力增强。
A feeding trial was eondueted to evaluate the maximum level of substitution of dietary fish meal by various proportions of soy protein coucentrate and peanut bran supplemented with coated lysine and methionine for Penaeus monodon. The shrimp （ mean initial weight 1.27 ±0. 02 g） were assigned to the 18 plastic cylinders（500 L） and ted with six diets containing 40% crude protein and 7% crude fat at a temperature of （30 ±1.0） ℃ for 56 days. The six test diets contained 30% （ F0, control）, 27% （FI）, 24% （ F2）,21% （F3）, 18% （F4）, 15% （F5） fish meal respectively. The results show that the shrimp fed with l＇Ud gain the maximum WG （245.02%） and SGR （2. 58%-d-t ） values, which are significantly higher than those fed with F3 - F5 （P 〈 0. 05 ）. The feed conver- sion ratio of the shrimp ted with F1 is the lowest and significantly lower than those fed with F3 - F5 （ P 〈 0. 05 ）. The survival rate of shrimp fed with F4 is the lowest and significantly lower than that fed with F0 - F2 （ P 〈 0. 05 ） , but without differences with other diets （ P 〉 0.05 ）. The diffrent level of fish meal substituted by plant proteins have no significant effects on the whole body composition of shrimp （ P 〉 0. 05 ）. The SOD activity in hepatopancreas of the shrimp fed with F2 is the highest and significantly higher than that of shrimp fed with F0 （ P 〈 0. 05 ）, but without differences with other diets （ P 〉 0. 05 ）. The activity of SOD in muscle is the highest in F5 and significantly higher than any other groups （ P 〈 0. 05 ）. The activity of ACP in muscle of the shrimp fed with F1 is the highest and significantly higher than those of shrimp fed with F2 - F4 （ P 〈 0. 05 ）. The activity of LSZ in muscle is the highest in F4 and F5 and significantly higher than FO - F3 （ P 〈 O. 05）. Based on the data of weight gain and specific growth rate, the plant feedstuffs sup- plemented with coated amino acids can replace dietary fish meal in P. monodon by 20% , and the non-specific inmmnity of shrimp is enhanced with the increase of the dietary plant proteins.
出处
《南方水产科学》
CAS
CSCD
北大核心
2013年第5期44-50,共7页
South China Fisheries Science
基金
广东省海洋渔业科技推广专项(A201201B02)
中央级公益性科研院所基本科研业务费专项资金(中国水产科学研究院南海水产研究所)资助项目(2011YD01,2010YD02)
现代农业(虾)产业技术体系建设专项资金(CARS-47)
关键词
斑节对虾
植物蛋白
鱼粉
生长性能
非特异性免疫力
Penaeus monodon
plant proteins
fishmeal
growth pertbrmance
non-specific immunity
