摘要
目的:建立黄芪药材的HPLC-DAD-ELSD色谱指纹图谱,采用相似度计算和化学模式识别的方法评价黄芪药材质量。方法:采用HPLC-DAD-ELSD串联技术,以Agilent Zorbax ODS C18色谱柱(4.6 mm×250 mm,5μm),乙腈-0.2%甲酸溶液为流动相进行梯度洗脱,流速0.8 mL·min-1,柱温25℃,进样量10μL,检测波长280 nm;ELSD汽化室温度110℃,氮气流速3.0 L·min-1。并对黄芪药材指纹图谱进行相似度计算和化学模式识别分析。结果:黄芪药材色谱指纹图谱测定方法稳定性、精密度和重复性良好,以18批黄芪药材建立指纹图谱共有模式,黄芪药材相似度均达到0.83,模式识别分析结果与其一致。结论:指纹图谱结合化学模式识别分析技术可以有效区分黄芪药材及其相关样品,可用于黄芪药材的质量评价,为药材的全面质量控制提供科学依据。
Objective： To develop an HPLC-DAD-ELSD method for detecting the fingerprint of Astragali Radix and evaluate the quality through similarity calculation and chemical pattern recognition. Method： Separation was performed at 25 ~C on an Agilent Zorbax ODS Clseolumn（4. 6 mm x250 mm,5 ~m）. Gradient elution was performed with the mobile phases of aeetonitrile and water containing 0. 2% formic acid. The flow rate was 0. 8 mL ~ min-1 , and sample size was 10 ixL. The UV detection wavelength was set at 280 nm. The drift tube temperature for ELSD was set at 110 ~C, and the nebulizing gas flow rate was 3.0 L ~ min-1. The similarity calculation and chemical pattern recognition were used for fingerprint analysis. Result： The HPLC-DAD-ELSD method for chromato- graphic fingerprint of Astragali Radix showed better results of stability, precision and repeatability. The reference chromatographic fin- gerprint of Astragali Radix was established on the eighteen Astragali Radix samples from different sources. The results of similarity cal- culation were higher than 0. 83, which was in accordance with the result of chemical pattern recognition analysis. Conclusion： Finger- print and chemical pattern recognition analysis could effectively distinguish Astragali Radix from different source, which could be ap- plied to the quality control of Astragali Radix.
出处
《中国中药杂志》
CAS
CSCD
北大核心
2013年第19期3319-3323,共5页
China Journal of Chinese Materia Medica
基金
国家"重大新药创制"科技重大专项(2009ZX09308-003)
