摘要
采用伤根法对3种常用桉树无性系组培苗进行初步的抗青枯病性能测定。结果表明,3种不同桉树无性系抗性强弱差异显著,DH32-29为中感品系,DH32-27和H1为高感品系。采用打孔法和浸根法测定了生物制农药多粘类芽孢杆菌细粒剂对桉树青枯病病原菌的室内拮抗作用和盆栽生防效果。结果表明:生物制剂农药多粘类芽孢杆菌细粒剂对桉树青枯菌具有较显著的抑菌效果,培养48 h后抑菌圈平均直径达到10.3 mm;浸根法处理后不同桉树无性系组培苗青枯发病率均有一定程度的降低,生防效果随着无性系的抗性增加而升高。总体来看,多粘类芽孢杆菌细粒剂对桉树组培苗青枯病的发生有一定的防治作用。
The disease resistance of the tissue culture seedlings of eucalypt against bacterial wilt caused by Ralstonia solanacearum was determined by the wounded roots dipped. The test results showed that the resistance ability of different clones of eucalyptus was significant difference. DH32-29 was susceptible clones; DH32-27 and H1 were highly susceptible clones. The biocontrol effect of Paenibacillus polymyxa was determined by the agar-well diffusion method in vitro and soaking root in greenhouse, and demonstrated that the average diameter of inhibition circle reached 10.3 mm at 30 ~C for 48 h. Three clones of the tissue culture seedlings of eucalypt in pots were tested in greenhouse. The incidence rates were somewhat lower and the biocontrol effect was higher in resistant clones than in susceptible ones. In summary, P. polymyxa had obvious controlling effect on bacteria wilt of the tissue culture seedlings of eucalypt.
出处
《热带作物学报》
CSCD
北大核心
2013年第9期1786-1790,共5页
Chinese Journal of Tropical Crops
基金
拮抗青枯病菌菌株的筛选及应用技术(No.2012BAD19B08)
桉树重大病虫害控制技术研究与示范(No.2010kjcx015-03)
关键词
桉树青枯病
抗病性
多粘类芽孢杆菌
生物防治
Bacterial wilt in eucalyptus
Disease resistance
Paenibacillus polymyxa
Biocontrol
