摘要
以三氯化铁和壳寡糖为原料,合成了壳寡糖铁配合物,产率为55.56%。原子吸收光谱法测定其中铁含量为27.60mg/g。利用紫外-可见光谱、红外光谱两种表征手段,证实了壳寡糖铁配合物的合成。采用邻苯三酚自氧化法、DPPH法测定壳寡糖铁配合物的抗氧化能力。结果表明,在实验设置的浓度范围内,壳寡糖铁配合物的抗氧化能力随着浓度的增加而增加,且壳寡糖铁配合物的抗氧化能力高于壳寡糖。2.0mg/mL的壳寡糖铁配合物对超氧阴离子自由基的清除率为59.84%,对DPPH自由基的清除率为65.76%。
Ferric trichloride and chitooligosaccharide were used to synthesize chitooligosaccharide-Fe complex in this experiment by chemical method. The yield was 55.56%. The content of Fe was 27.60mg/g measured by atomic absorption spectrometry method. Chitooligosaccharide-Fe complex was characterized by visible ultraviolet （UV-VIS） and infrared （IR） techniques. The antioxidant activities in vitro of chitooligosaccharide-Fe complex were investigated by pyrogallol autoxidation and DPPH method. The results showed that in the setting concentration range,the antioxidant activities increased with the elevation of concentration. The antioxidant activities of chitooligosaccharide-Fe complex were better than that of chitooligosaccharide. The clearance rates of superoxide anion free radical and DPPH of 2.0mg/mL chitooligosaccharide-Fe complex were 59.84% and 65.76%.
出处
《食品工业科技》
CAS
CSCD
北大核心
2013年第20期119-121,共3页
Science and Technology of Food Industry
基金
国家自然科学基金(31172009)
辽宁省教育厅项目(L2013510)
中央高校基本科研业务费专项资金资助项目(DC110318)
大连民族学院大学生创新创业训练计划资助项目(X2013033)
大连民族学院"太阳鸟"项目
关键词
壳寡糖
壳寡糖铁配合物
合成
表征
抗氧化性
chitooligosaccharide
chitooligosaccharide-Fe complex
synthesis
characterization
antioxidant activity
