摘要
目的应用基因芯片分析技术,筛选乳腺癌多药耐药细胞系相关基因,探讨乳腺癌化疗耐药机制。方法阿霉素持续递增作用于MCF-7细胞株,建立多药耐药细胞系MCF-7/ADM;利用Human Expr 12x135K Arr Del芯片检测MCF-7/ADM与MCF-7的差异表达基因,并利用荧光定量PCR验证差异最为显著的基因。结果不同的细胞系中筛选出差异有显著性意义的基因1520个,其中MDR1、p170、p65皆为耐药途径中较为关键的几个蛋白,p170与p65主要表现在蛋白活性的差异上,MDR1的高表达则是耐药株的显著特征。结论乳腺癌耐药涉及多种途径、多个基因,基因芯片分析技术可为乳腺癌多药耐药细胞系相关基因提供全面的技术分析。
Objective To screen multi - drug resistance gene in breast cancer by gene chip technology, so as to explore the drug resistance mechanism of breast cancer. Methods The concentration of doxorubicin was continuously increased in MCF - 7 cell line to establish a multi -drug resistant cell line MCF -7/ADM. Human Expr 12x135K Arr Del was used to detect the differentially - expressed genes of MCF -＇7/ADM and MCF - 7. Fluorescence quantitative PCR was employed to verify the most obvious differentially - expressed gene. Results There were 1520 differential - expressed genes in MCF - 7/ADM and MCF - 7 cell lines. MDR1, p170 and p65 were critical proteins in resistant pathway. P170 and p65 is mainly manifested in protein ac- tivity. High expression of MDR1 was a significant feature of the drug - resistant strains. Conclusion Multi - drug resistant breast cancer involves a variety of ways and multiple genes. The gene chip analysis technology can provide comprehensive sup- port for muhidrug resistance in breast cancer cell lines .
出处
《中国卫生检验杂志》
北大核心
2013年第11期2477-2480,共4页
Chinese Journal of Health Laboratory Technology
基金
杭州市科技局基金资助(20100633B16)
关键词
基因芯片
阿霉素
乳腺癌
耐药
Gene chip
Adriamycin
Breast cancer
Drug resistance
