摘要
本试验以鲤鱼TLR5M的EST序列为基础进行5'-RACE试验,获得了其cDNA的全长序列。结果表明,该序列共3182 bp,包含38 bp的5'端非编码区,486 bp的3'端非编码区,1个2658 bp的开放阅读框(ORF),共编码885个氨基酸。序列同源性比对结果表明,该序列与麦瑞加拉鲮鱼TLR5基因同源性高达84.46%。
In this paper, we have isolated, sequenced and characterized the full-length cDNA of TLR5M gene from common carp by the methods of rapid amplification of cDNA ends（RACE） on the basis of its EST sequences. The results showed that the TLR5M gene was 3182 bp in length,vthe gene consists of a 38 bp 5＇ untranslated region（UTR）, 486 bp 3＇UTR and a 2658 bp open reading frame（ORF）, encoding a peptide of 885 amino acids. The amino acid sequence of carp TLR5 gene shared 84.46% identity with cirrhinus mrigala.
出处
《中国畜牧兽医》
CAS
北大核心
2013年第9期41-45,共5页
China Animal Husbandry & Veterinary Medicine
基金
国家自然科学基金(30972277)
