摘要
目的利用基因芯片技术初步筛选对电离辐射剂量敏感的血清细胞因子并分析其变化规律。方法健康雄性C57BL/6小鼠80只,随机分为对照组(n=20)和辐射组(n=60)。辐射组大鼠分别接受剂量为3、5、10Gy的60Co一次性照射,每个剂量组20只。照射后6h经眼球取血,留存血清。采用基因芯片技术检测血清细胞因子基因的表达水平,筛选出有变化的细胞因子,分析其变化规律。结果共筛选96种细胞因子。当辐射剂量为3Gy时,有44种细胞因子表达发生变化,当辐射剂量为5Gy和10Gy时,分别有47种和66种细胞因子表达发生变化。进一步分析发现,有14种因子表达呈剂量-效应依赖,其中7种随剂量上升表达增加,7种随剂量上升表达下调。结论通过基因芯片技术筛选的辐射敏感的细胞因子,其随辐射剂量的变化存在一定规律,这种规律对估算电离辐射剂量有一定意义。
Objective To identify the cytokine biomarker candidates in serum associated with ionizing radiation dose, and analyze the patterns of them by cytokine antibody array. Methods Eighty male C57BL/6 mice were randomly divided into radiation group （n=60） and control group （n=20）. Mice in radiation group were treated with 60Co total-body irradiation with the dose of 3, 5 and 10Gy, respectively （20 each dose group）. Six hours after radiation, blood was obtained from the orbital sinus bleeding, and serums were collected. The serum cytokine concentration was analyzed by antibody array of 96 mouse cytokines, and cytokine biomarker candidates which were shown to be dose-responsive to ionizing radiation had been identified. Results Interestingly, the majority of the 96 mouse cytokines showed positive changes in amounts of cytokine. Following ionizing radiation exposure, only 44 cytokines changed at the expose dose of 3Gy, while 47 cytokines changed at the dose of 5Gy and 66 cytokines at the dose of 10Gy. Further analysis revealed that 14 cytokines were shown to be dose-responsive to ionizing radiation. Among those, 7 cytokines were shown up-regulated in a dose-dependent trend in response to ionizing radiation, and 7 cytokines were shown down-regulated. Conclusion These serum cytokine biomarkers which are sensitive to ionizing radiation may act in a dose-dependent manner, suggesting that the serum cytokine biomarker candidates can be used for estimating dose of ionizing radiation.
出处
《解放军医学杂志》
CAS
CSCD
北大核心
2013年第10期822-825,共4页
Medical Journal of Chinese People's Liberation Army
基金
上海市科学技术委员会医学引导类科研项目(10411969300)
总后"十一五"科技攻关项目(08G037)
总后科研项目(AHJ09J012)
上海市卫生局项目(20124435)~~
关键词
辐射
电离
细胞因子类
辐射剂量
radiation, ionizing
cytokines
radiation dosage
