摘要
利用基因重组技术,以枯草杆菌表达载体pWB980为骨架,插入大肠杆菌的克隆载体pUC18中的抗性基因和复制子,构建穿梭质粒pY980coli。将海洋细菌蛋白酶基因hspa插入pY980coli载体P43启动子下游,导入枯草杆菌WB600中,可实现活性表达。
A versatile Escherichia coli-Bacillus subtilisshuttle vector pY980coli was constructed by genetic recombination.Resistance gene and replicon of pUC18,a cloning vector forEscherichia coli,were integtated into theBacillus subtilisexpression plasmid pWB980.Protease genehspafrom a marine bacterium was then inserted under P43 promoter of pY980coli,and the recombinant vector was transformed intoBacillus subtilisWB600.Protease activity was detected in the fermentation broth.
出处
《生物技术通报》
CAS
CSCD
北大核心
2013年第9期146-150,共5页
Biotechnology Bulletin
基金
国家“973”计划项目(2010CB833804)
国家“863”计划项目(2012AA092104)
海洋公益性行业科研专项(201305018)
