摘要
为高效转化米糠生产食用菌粗多糖,拟采用猴头菌发酵米糠得到猴头菌丝原料。以米糠为基础培养基进行猴头菌液体发酵,在对培养基成分和发酵条件进行单因素实验的基础上,采用响应面法对米糠浓度、蛋白胨添加浓度、装样量三个主要因素进行优化。优化实验的其他控制参数为:葡萄糖20g/L、培养基pH5.0、KH2PO41.5g/L、MgSO40.75g/L,接种量10%、培养温度28℃、培养时间7d,得到优化条件为:250mL三角烧瓶装样150mL,蛋白胨浓度9.8g/L,米糠浓度23.3g/L,实验结果猴头菌菌丝干重为(11.28±0.082)g/L。
To make efficient use of the rice bran and to get mushroom mycelium polysaccharides,Hericium erinaceum was used to ferment rice bran to product polysaccharides. Hericium erinaceum was fermented in liquid medium with extract of rice bran and the medium components and culture conditions were investigated through single-factor experiment. By Box-Behnken experiments ,in which Glucose 20g/L, KH2PO4 1.5g/L, MgSO4 0.75g/L,inoculation volume 10% ,pH5.0,culture time 7d,temperature 28℃ were fixed,concentration of rice bran,peptone conoentration and loading volume were optimized. The optimal value were 23.3g/L,9.8g/L and 150mL（250mL flask） respectively. The optimal dry weight of mycelium was 11.28g/L.
出处
《食品工业科技》
CAS
CSCD
北大核心
2013年第18期192-196,共5页
Science and Technology of Food Industry
基金
江苏省社会发展科技计划项目(BS2006037)
江苏镇江市农业支撑科技项目(NY2008043)
江苏高校优势学科建设工程(PAPD)资助项目(2012年)
南京高源环保工程有限公司提供横向课题(JSU2011029)
关键词
猴头菌
米糠
发酵
响应面实验
Hericium erinaceum
rice bran
fermentation
response surface experiment
