摘要
目的研究三氧化二砷及联合紫杉醇对胃癌细胞株MGC803的生长和耐药性变化。方法 MTT法观察MGC803细胞的生长变化;免疫组化检测p-gp,bcl-2蛋白的表达;电镜观察超微结构变化。结果随着As2O3或联合紫杉醇作用时间的延长,对肿瘤细胞的抑制率逐渐增强,除0.5μmol/L As2O3作用外,其余各组随药物浓度的升高,其抑制率就越高,与对照组比较有明显差别(P<0.05),且同一As2O3浓度下联合用药对肿瘤细胞的抑制作用均强于单一用药(P<0.05);免疫组化分析显示As2O3或联合用药能同时下调p-gp和bcl-2蛋白;电镜下肿瘤细胞出现凋亡改变,并伴有坏死。结论三氧化二砷对人胃癌MGC803细胞具有明显的抑制作用,同时能够通过下调p-gp,bcl-2蛋白的表达,并且与紫杉醇具有协同作用,延缓耐药性的发生。
Objective To study growth and drug resistance changes of gastric cancer cell MGC803 with arsenious acid and compound of As203 and paclitaxel. Methods MTT method was used to observe growth changes of MGC803 cell; Immunohisto chemical method was used to measure p-gp,bcl-2 protein expression and electron- microscopy was used to observe uhrastructural changes. Results The inhibition ratio of tumor cell was gradually increased with the longer extension time of As：03 or compound of AszO3 and paclitaxel. Excepted the group of 0.5 μmol/L As203, the other groups inhibition ratios were highter with the increase of drug concentration on the third day which was significant difference （P〈0.05）compared with the control group. The inhibition ratio of combination drugs was stronger than that of single drug with the same concentration of As2O3 （P〈0.05）. Immunohistochemistry showed that As203 could downregulate the expression of p-gp and bcl-2.Under the electron microscope ,the tumor cells appeared apoptosis and necrosis changes. Conclusion As2Os can significantly inhibits human gastric cancer MGCS03 cells, meantime down-regulate of p-gp,bcl- 2 protein expression especially has synergistic effect combined with paclitaxel,can delayed drug-resistance occuring.
出处
《中国现代医生》
2013年第29期4-6,10,共4页
China Modern Doctor
基金
黑龙江省卫生厅科技计划立项课题(2011-272)
