摘要
目的 提高毛细管电泳法的灵敏度,建立测定中成药中微量士的宁的高效毛细管电泳法.方法 采用高效毛细管电泳场放大富集法,运行缓冲液为70 mmol· mL-硼砂-乙腈（83：17,pH5.5）,10 kV电动进样,进样时间16 s,分离电压25 kV,分离温度25 c℃,检测波长205 nm.结果 士的宁0.2～16.0 μg·mL-1与峰面积的线性关系良好（r=0.9987）,检测限为0.022μg·mL-1.结论 所用方法准确、简便,有效提高了毛细管电泳法的灵敏度.
OBJECTIVE To improve the sensitivity of capillary electrophoresis and establish an HPCE method for determination of the trace strychnine in Chinese patent medicine. METHODS Field - amplified capillary electrophoresis was used with the running buffer of 70 mmol·mL-1 boric acid- acetonitrile（83 ： 17）（pHS. 5） and an applied voltage of 25 kV at 25℃. The detection was performed with diode array detection at 205 nm. The sample was eventually diluted by ultra - pure water and injected for 16 s in electrokinetic type. RESULTS The linearity of strychnine was obtained over the rang of 0.2～16.0 μg·mL-1 （ r = 0. 9987 ） and the detection limit was 0.022μg·mL-1. CONCLUSION The method is simple,sensitive and effectively improved the sensitivity of capillary electroDhoresis.
出处
《华西药学杂志》
CAS
CSCD
北大核心
2013年第5期509-511,共3页
West China Journal of Pharmaceutical Sciences
基金
国家"十一五"科技支撑计划课题资助(课题号:2006BAD27B02)
关键词
高效毛细管电泳场放大
中成药
士的宁
Field - amplified capillary electrophoresis
Chinese patent medicine
Strychnine
