摘要
目的:优化酶解血渣中提取血红素的最佳提取条件。方法:取酶解制备血红蛋白肽的血渣为原料,采用酸性丙酮法,用L9(34)正交实验,对影响提取效果的因素(盐酸丙酮比A、料液比B、搅拌时间C)进行优选。结果:血渣中提取血红素的最佳工艺条件是盐酸丙酮比为4∶100、料液比为1∶5、搅拌时间为20min,提取的血红素含量可以达到97%以上,铁含量7.8%以上,提取率可达到62.89%。结论:此工艺易于操作、重复性好、所得产品纯度高,适合工业化生产。
Objective：To optimize the extraction process of hemin in the enzymatic hydrolysis blood residue.Method ： Taking the enzymatic hydrolysis blood residue of preparation hemoglobin peptide as raw material,the hydrochloric acid consumption A, solvent consumption B, mixing time C were optimized by the L9 （34 ） orthogonal design. Results.The optimum extration condition were as follows= hydrochloric acid to acetone ratio = 4：100,5 folds of Acidic acetone,stirring 20min.The purity of extracted hemin could reach more than 97%, the iron conten could reach more than 7.8%.Conclusion.This method was easy to operate, had better reproducible and could be used for industrial production.
出处
《食品工业科技》
CAS
CSCD
北大核心
2013年第21期206-209,214,共5页
Science and Technology of Food Industry
关键词
渣
血红素铁
提取
铁含量
blood residue
heme iron
extraction
iron conten
