摘要
为研究葛根黄酮的提取工艺,对多个影响因素进行了单因素考察,并选择乙醇质量分数、提取时间、液固比与提取温度4个因素采用响应面法进行了优化,建立了数学模型并进行了方差分析。结果表明,所得模型能很好地描述提取率与各因素之间的关系,黄酮提取的最佳工艺为:乙醇质量分数63.9%、提取时间138.0min、液固比11.3mL/g、提取温度72.9℃、1次提取;提取时溶液pH值不需调整;乙醇回收循环使用可行。采用该工艺条件,黄酮理论提取率可达到1 842.6μg/g,实际为1 821.3μg/g。
The muhiple influence factors were investigated through the single factor for researching the extraction technology of flavonoids from radix pueraria. Four factors of quality fraction of ethanol, extraction time, liquid-solid ratio and extraction temperature were chosen to optimize with response surface methodology（RSM）. A mathematical model was established and the analysis of variance was carried out. The result shows that the obtained model is significant and the optimum extraction conditions are as follows： 63.9 %0 of quality fraction of ethanol, 138.0 min of extraction time, 11.3 mL/g of liquid-solid ratio, 72.9 ~C of extraction temperatui＇e and one time extraction; when extracting the pH value of solution does not need to adjust; the recycle use of ethanol is feasible. Using the process conditions, the theoretical extraction yield of flavonoids is 1 842.6 /~g/g; the practical extraction yield is 1 821.3 ~g/g.
出处
《浙江科技学院学报》
CAS
2013年第5期366-372,共7页
Journal of Zhejiang University of Science and Technology
关键词
葛根
黄酮
提取工艺
响应面法
工艺优化
radix pueraria
flavonoids~ extraction technology
response surface methodology
technology optimization
