摘要
采用不同月龄的乌珠穆沁羊骨骼肌中的股二头肌,采取组织化学染色法对肌纤维类型的变化进行研究,了解肉羊生长过程中肌纤维类型的变化规律。骨骼肌用多聚甲醛固定液固定、切薄片。在酸性和碱性孵育液中孵育后,巴比妥钠溶液中冲洗后硫化铵液中孵育。用乙醇脱水,中性树胶封片,显微镜下观察。结果表明,随着月龄的增加,股二头肌中Ⅱa型肌纤维百分含量在3、6月龄及12、18月龄之间差异不显著(P>0.05),百分含量在12月龄时最高。Ⅱb型肌纤维的百分比在18月龄最高。I型纤维在18月龄时含量最低。
Changes in muscle fiber types in M.biceps femoris of Ujumqin sheep at different moths of age were examined by histochemical staining.The aim of the experiment was to explore the changing regularity of muscle fiber types during the growth of Ujumqin sheep.The skeletal muscle was fixed in paraformaldehyde fixative solution and histological slides were prepared and incubated separately in acidic and alkaline incubation solutions,then washed with barbital sodium buffer and placed in（NH4）2S solution for further incubation.histological slides were dehydrated with gradient ethanol and finally mounted with neutral balsam prior to microscopic observation.Results showed that the percentage of type Ⅱa muscle fiber in the M.biceps femoris exhibited no significant difference between 3 and 6 months as well as between 12 and 18 months of age（P＆gt;0.05） and the highest level was observed in the M.biceps femori of 12-year-old sheep.Type Ⅱb muscle fiber showed the highest percentage in the M.biceps femori of 18-year-old sheep,which,on the contrary,showed the lowest percentage of type Ⅰ muscle fiber.
出处
《肉类研究》
2013年第7期10-13,共4页
Meat Research
基金
国家自然科学基金地区科学基金项目(20766003)
内蒙古自然科学基金项目(2010MS1304)
关键词
乌珠穆沁羊
骨骼肌
肌纤维类型
十二烷基硫酸钠聚丙烯酰胺凝胶电泳
Ujumqin sheep
skeletal muscle
muscle fiber type
sodium dodecyl sulfate polyacrylamide gel electrophoresis（SDS-PAGE）
