摘要
利用16S rRNA基因克隆文库技术探索中国浓香型白酒窖池上、中、底三层窖泥中原核微生物群落构成的异质性和系统发育关系。结果表明:窖池不同层面的窖泥中细菌类群及数量的差异性较大。其中,优势菌群Clostridium diolis及Lactobacillus acetotolerans在上、中、底层窖泥中所占比例分别为34.9%和39.8%、25.2%和20.6%、23.8%和36.6%,Bacillus subtilissubsp.subtilis是中层窖泥优势菌群之一,占22.1%;古细菌群落主要分布在Methanoculleus属和Methanosarcina属。其中,Methanoculleus bourgensis在上层和底层窖泥中所占比例分别为89.9%和88.2%。Methanosarcina siciliae和Methanoculleus bourgensis在中层窖泥中所占比例分别为40.7%和49.1%。系统发育分析显示:窖泥细菌主要为杆菌目、梭菌目、肠杆菌目及以TM7 phylum sp.为代表的种属分类尚不明确的细菌,古细菌主要为甲烷微菌目与甲烷八叠球菌目。
The heterogeneity of community structure and the phylogeny of prokaryotic microorganisms in pit mudcollected from Luzhou-flavor liquor fermentation cellar in different regions were detected by 16S rRNA cloning technique.The results showed that significant variability of the composition and quantity of bacterial communities wasobserved in pit mud. The populations of Clostridium diolis and Lactobacillus acetotolerans as two dominant communitiesfrom the top layer, middle layer and bottom layer were 34.9% and 39.8%, 25.2% and 20.6%, and 23.8%and 36.6%, respectively. In the pit mud, Bacillus subtilis subsp. subtilis was also one of the dominant communities,and had a population of 22.1% in the middle layer. The community of archaea was mainly constructed withMethanoculleus sp. and Methanosarcina sp. Methanoculleus bourgensis was the dominant community in the top layerand the bottom layer with a population of 89.9% and 88.2%, respectively. Methanosarcina siciliae and Methanoculleusbourgensis were the dominant communities in the mid layer with a population of 40.7% and 49.1%, respectively.Phylogenetic analysis showed that Bacilli, Clostridiales, Enterobacteriales and some other bacteria such asTM7 phylum sp with unclear classification were the main species of bacteria in the pit mud. Methanomirobiales andMethanosarcinales were the main archaea in the pit mud.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2013年第21期221-226,共6页
Food Science
基金
教育部春晖计划项目(Z2010101)
四川省食品生物技术重点实验室开放基金项目(SZJJ2009-014)
西华大学重点科研基金项目(Z0910501)
