摘要
[目的]比较水稻胡麻斑病菌（B-1）和水稻纹枯病菌（T-3）在不同培养基上的回收率.[方法]采用查理琼脂（RA）、马铃薯葡萄糖琼脂（PDA）和查彼琼脂（CA）3种培养基分别培养2种病菌,然后测定其回收率.[结果]2种病原菌回收率范围为13.47％～ 75.26％,其中胡麻斑病菌的平均回收率较高,达65.48％,纹枯病菌的平均回收率较低,仅为29.85％.同一菌株在不同培养基上的回收率也有所差别,胡麻斑病菌在RA上的回收效果最好,回收率达71.51％,而在PDA和CA上较差;PDA对纹枯病菌的回收效率相对较高,达到48.49％.[结论]RA和PDA可分别作为检测水稻胡麻斑病菌和水稻纹枯病菌的基础培养基.
[ Objective ] The aim was to study effects of different culture media on coefficient of recovery （CR） of rice brown spot germ and rice sheath blight germ. [ Method] Richard＇ s agar, PDA and Czapek＇ s agar were used to detect the different CR of the two species of germ. [ Result] CRs were different from 13.47% to 75.26%, and average CR was 65.48% for B-1 and 29.85% for T-3, respectively. There was significant difference of CR on different media for the same strain. B-1 had the highest CR of 71.51% on RA, and fewer on PDA and CA. CR ofT-3 was 48.49%. [ Conclusion~ RA and PDA could be used as the basal media for rice brown spot germ and rice sheath Night germ.
出处
《安徽农业科学》
CAS
2013年第23期9804-9804,9819,共2页
Journal of Anhui Agricultural Sciences
基金
湖北省教育厅科学研究计划项目(Q20122802
B2013050)
湖北科技学院国家级大学生创新创业训练计划项目(201210927025)
关键词
水稻胡麻斑病
水稻纹枯病
培养基
回收率
Rice brown spot
Rice sheath blight
Culture media
Coefficient of recovery
