摘要
目的:国标中VA、VD的测定特别是VD比较繁琐,故将其优化。方法:试样经皂化后用石油醚提取,提取过程改用摇床振摇的方式,另外取消了VD3的正相收集,VA、VD直接反相色谱分离,外标法定量。结论:通过线性考察和回收率试验,证明此方法准确可靠。结果:此方法适用于VD3含量较高的样品,操作起来准确快速。
Objective： National standard in the determination of VA, VD, especially VD is tedious, so to optimize it. By petroleum ether extraction. Method： Sample after saponification, extraction process using the bed vibration wave, also cancelled the VD3 is collected, VA, VD reversed-phase chromatographic separation directly and external standard method. Conclusion： Through linear investigation and recovery experiments show that this method is accurate and reliable. Results： This method is suitable for samples of higher levels of VD3, to operate quickly and accurately.
出处
《食品科技》
CAS
北大核心
2013年第11期276-279,共4页
Food Science and Technology
关键词
国标
鳕鱼肝油
VA
VD
高效液相色谱
GB
cod liver oil
VA, VD
high performance liquid chromatography
