摘要
采用人工土壤培养法,通过14 d急性暴露试验,研究了全氟辛烷磺酸(PFOS)对赤子爱胜蚓(Eisenia fetida)的死亡率、谷胱甘肽硫转移酶(GST)和过氧化氢酶(CAT)活性以及DNA损伤的影响。研究结果表明:PFOS对蚯蚓14 d-LC50为478.0 mg·kg-1,显示为低毒;急性暴露期间,PFOS显著抑制蚯蚓生长,生长抑制率随着PFOS浓度增加而增大,且有良好的剂量效应关系(r=0.951,P<0.01);PFOS对蚯蚓GST和CAT活性均没有显著性影响,没有观察到剂量效应关系;PFOS可致蚯蚓体腔细胞DNA损伤,不同浓度的PFOS对蚯蚓体腔细胞尾长、尾部DNA含量和尾矩的影响具有一定的剂量效应关系(P<0.01),与对照组之间存在显著性差异(P<0.05)。上述结果表明,PFOS对蚯蚓的存活影响显示为低毒,但是对生长有一定影响,可致蚯蚓体腔细胞DNA损伤。
Artificial soil method was applied to study the effects of perfluorooctane sulfonate （PFOS） on earthworms （Eiseniafetida） during 14 d exposure. Survival, activities of glutathione S-transferase （GST）, cata- lase （CAT） and DNA damages of earthworms were detected. The results showed that 14 d-LC50 was 478.0 mg· kg^-11, indicating low toxicity;PFOS significantly inhibited the growth of earthworms during acute exposure, and the growth inhibition rates increased with the increase of PFOS concentration, showing dose-response re- lationship （r=0.951,P 〈0.01）. Neither GST nor CAT activity showed dose-response relationship with PFOS concentration; DNA damages of earthworm coelomocytes were detected after 14 d exposure to PFOS. Tail length （TL）, tail DNA content （TD） and tail moment （TM） values not only exhibited a significant difference （P 〈0.05） compared with the control, but also had a strong dose-response relationship （P 〈0.01）. In conclusion, PFOS had a low toficity effect on survival of earthworms; however, it could inhibit the growth, and damagethe coelomocytes DNA of earthworms.
出处
《生态毒理学报》
CAS
CSCD
北大核心
2013年第5期687-694,共8页
Asian Journal of Ecotoxicology
基金
国家自然科学基金(41271487)
国家国际科技合作专项(2012DFA91150)
