摘要
目的通过DNA微阵列芯片法进行分枝杆菌菌种鉴定与常规生化法进行比较,分析其特点,提高分枝杆菌菌种鉴定水平,更好的为临床服务。方法选择我院2010年1月至2013年3月从临床标本中分离培养后所得的结核分枝杆菌复合群12株(其中含1株牛型结核分枝杆菌),非结核分枝杆菌31株,分别用DNA微阵列芯片法和常规生化法进行鉴定。结果 DNA微阵列芯片法进行分枝杆菌菌型鉴定与普通生化培养法鉴定结果符合率为100%,对常规生化法未定型的两株非结核分枝杆菌也分别定型为1株土分枝杆菌,1株耻垢分枝杆菌。结论 DNA微阵列芯片法与常规生化法相比在分枝杆菌菌型鉴定中具有更快速、准确的特点,是分枝杆菌菌型鉴定的有利工具。
Objective To compare DNA microarray method and conventional biochemical method in identification of Mycobaeterium species, Analyse their characteristics, improve the identification of Mycobaeterium species level, eontrib ute to clinical services. Methods 12 strains of Mycobacterium tuberculosis complex group （including 1 strain of Myco- bacterium bovis）,33, strains of non Mycobacterium tuberculosis,isolated from clinical specimens from 2010 January to 2013 March in our hospital, respectively identificated, by DNA microarray method and conventional biochemical meth od. Results Two strains of non Myeobacterium tuberculosis undefined in conventional biochemical method were identi lied by DNA microarray ,one was a strain of Mycobacterium terrae ,the other was a strain of Mycobacterium smegma tis. Conclusion DNA microarray method was more rapid and accurate than conventional biochemical method in identifi- cation of Mycobacterium species. , It was an useful tool for identification of Mycobacterium species.
出处
《中国实验诊断学》
2013年第11期2026-2029,共4页
Chinese Journal of Laboratory Diagnosis
关键词
DNA微阵列
分枝杆菌
结核分枝杆菌复合群
非结核分枝杆菌
核酸杂交
DNA Microarray
Mycobacterium
Mycobaeterium Tuberculosis complex group
Nontuberculous My cobacteria
Nuleotide hybridization
