摘要
为研究不同中药复方对大肠杆菌、沙门氏菌2种临床耐药菌株R质粒的消除作用,以三黄汤等4种中药复方水煎剂为质粒消除剂,以临床分离的多重耐药大肠杆菌及沙门氏菌为靶细菌,采用肉汤二倍稀释法和影印培养法进行质粒消除试验。结果显示,4种中药复方对2种临床耐药菌R质粒均有消除作用(消除率0.7%～9.3%),不同中药复方耐药性消除子均丢失2～3个质粒条带(2 000～21 000bp),并丢失耐药表型。表明4种中药复方对耐药大肠杆菌和沙门氏菌R质粒均有消除作用。
To study the plasmid curing in Escherichia coli and Salmonella by different traditional Chinese medical（TCM ） compounds ,the R plasmid curing experiment was performed using 4 dif-ferent Chinese medical decoctions .The multi-resistant Escherichia coli and Salmonella isolates were used as target bacteria .The influence on R plasmid curing was observed by broth doubling dilution and replica plating method .The results showed that the 4 decoctions exhibited resistance curing effects against Escherichia coli and Salmonella ,the curing rates ranging from 0 .7% -9.3% and 2 or 3 plasmids ,2 000-21 000 bp-long ,were lost in different curing strains .The miss-ing patterns of R plasmid occurred as the disappearance of different resistant phenotypes .This in-dicates that the 4 different Chinese medical compounds have R plasmid curing effects against Escherichia coli and Salmonella .
出处
《河南农业科学》
CSCD
北大核心
2013年第11期149-153,共5页
Journal of Henan Agricultural Sciences
基金
四川优势产业发展急需紧缺专业博士后项目与教育厅科研项目(20100301)
四川农业大学双支计划项目(201303)
关键词
中药复方
大肠杆菌
沙门氏菌
R质粒
消除
traditional Chinese medical compound
Escherichia coli
Salmonella
R plasmid
curing
