摘要
覆盖在植物表面的蜡粉主要是由特长链饱和脂肪酸的衍生物组成的,它具有防止非气孔的水分散失、病虫害的侵入和太阳辐射的生物学功能。本研究以大白菜花茎上有蜡粉的"08A161"为母本,无蜡粉的"08A235-2"为父本构建了F2代定位群体。遗传分析表明,大白菜花茎有蜡粉性状受单一的显性基因控制,利用308个F2代隐性纯合单株,将蜡粉基因定位在A01连锁群上,根据锚定标记nia-m086a开发SSR引物,初步将目的基因定位在SSRzx45与SSRzx54标记之间,遗传距离分别为1.54 cM和0.56 cM。蜡粉基因的初步定位奠定了基因的精细定位和基因克隆的前期理论基础。
The surface of plants is covered with a cuticular wax, which contains a mixture of very long-chain fatty acid derivatives. This wax layer provides a hydrophobic barrier which reduces non-stomatal water loss, prevents pathogen attack and protects plants from ultraviolet radiation. In this study, we constructed F2 segregating popu- lation taking Chinese cabbage inbred lines ＇08A16 I＇ （waxy） and ＇08A235-2＇ （glossy） as paternal plants. Genetic analysis indicated that the waxy trait of stem was controlled by a single dominant gene. Using 308 F2 recessive glossy individuals, we mapped the wax gene to A01 of＇Brassica rapa linkage group and developed SSR markers according nia-m086a as anchored markers, the wax gene was mapped between SSRzx45 and SSRzx54, with a genetic distance of 1.54 cM and 0.56 cM, respectively. The preliminary mapping of wax gene would lay a basis for its fine mapping and gene cloning.
出处
《分子植物育种》
CAS
CSCD
北大核心
2013年第6期804-808,共5页
Molecular Plant Breeding
基金
国家自然科学基金(31272157
31201625)资助
关键词
蜡粉
SSRS
大白菜
Cuticular wax, SSRs, Chinese cabbage
