摘要
目的调查广东人群CYP2C9基因第3外显子374G>A位点多态性。方法应用创造酶切位点PCR扩增跨越374G>A位点的DNA片断,用MluI酶切PCR产物,通过PAGE-银染判断基因型。结果在受检的532例广东人中,未检出A374突变等位基因即CYP2C9*14等位基因,所有个体的基因型均为野生型纯合子G374/G374。结论 CYP2C9*14等位基因为广东人群的稀有等位基因。
Objective To investigate the polymorphism of G374A in exon 3 of CYP2C9 gene in Guangdong population. Methods The DNA fragments spanning the G374A locus in exon 3 of CYP2C9 gene were amplified by created restriction site PCR （CRS-PCR） ,the PCR products were subjected to MluI digestion, and then the genotypes were determined with polyacrylamide gel electrophoresis （PAGE） and sliver- staining. Results A374 allele, namely CYP2C9 ＊ 14, was not detected in 532 individuals studied. All individuals were wild-type homozygotes G374/G374. Conclusion CYP2C9 ＊ 14 is a rare allele in Guangdong population.
出处
《广东药学院学报》
CAS
2013年第5期536-538,共3页
Academic Journal of Guangdong College of Pharmacy
基金
2010年广东省大学生创新实验项目(1057310046)
广东省医学科研基金(A2008308)
广东药学院科研启动基金(2007jcx02)
关键词
CYP2C9
多态性
创造酶切位点
Cytochrome P450 2C9
polymorphism
created restriction site
