摘要
目的 探讨蛛网膜下腔出血（SAH）后大鼠血、脑脊液不对称二甲基精氨酸（ADMA）的浓度变化及其与一氧化氮（NO）浓度和脑血管痉挛（CVS）的关系.方法 将78只SD大鼠分为对照组（n=6）、假手术组（n=36）及SAH组（n=36）,采用枕大池二次注血法建立SAH动物模型.定期检测各亚组血浆、脑脊液中ADMA、NO浓度;显微镜下测量基底动脉壁厚度及动脉内径.结果 （1）SAH组脑脊液ADMA浓度逐渐升高,至术后5d达其最高值;NO浓度逐渐降低,至术后5d达其最低值;（2）以基底动脉壁厚度与内径的比值作为评价CVS程度的指标并进行Pearson相关性分析,SAH组脑脊液ADMA浓度与CVS程度呈正相关;与脑脊液NO浓度呈负相关.（3） SAH组血浆ADMA浓度无明显变化.结论 ADMA可能通过抑制NO生成影响SAH大鼠CVS的发生与发展;脑脊液ADMA水平与SAH大鼠CVS程度明显相关,可作为预测CVS的指标;血浆ADMA水平与CVS程度无明显相关.
Objective To investigate the correlation between asymmetric dimethylarginine （ADMA） and cerebral vasospasm （CVS） secondary to subarachnoid hemorrhage （SAH）.Methods Seventy-eight healthy male adult Sprague-Dawley rats were randomly allocated into control group （n =6）,sham group （n =36） and SAH group （n =36）.In SAH group,a SAH model was established by double autologous blood injection into cisterna magna.Arterial blood,cerebrospinal fluid （CSF） and basilar artery samples of each group were collected at the preconcerted time.ADMA levels in the plasma and CSF samples were assessed using enzyme-linked immuno sorbent assay （ELISA）.Nitrite and nitrate concentrations were used as a marker of nitric oxide production in plasma and CSF samples and determined by using nitrate reductase method.Pathomorphological changes were observed with optical microscope and inner diameter of basilar artery was measured by Micrometer after hematoxylin-eosin staining was performed.Results （1） In SAH group,ADMA levels in CSF samples were significantly increased at 1st,3rd,5th and 7th day （P ＜0.05）,and NO levels in CSF samples were significantly decreased at 3rd,5th,and 7th day as compared with sham group （P＜0.01）.（2） The ratio of thickness and inner diameter of basilar artery was used to indicate the degree of CVS.The level of ADMA in CSF samples was strongly correlated with the degree of CVS （r =0.641,P ＜0.01）.Nitrite levels in CSF samples were negatively correlated with ADMA levels in CSF samples （r =-0.687,P ＜0.01）.（3） Plasma ADMA levels in sham group and SAH group were similar and remained essentially unchanged during the several days after SAH.Plasma ADMA levels were neither correlated with NO levels （r =0.019,P ＞ 0.05） nor with the degree of vasospasm （r =-0.046,P ＞ 0.05）.Conclusion Asymmetric dimethylarginine reduces the level of nitric oxide in CSF by inhibiting nitric oxide synthase and may be involved in the progression of cerebral vasospasm.The level of ADMA in CSF samples was strongly correlated with the degree of CVS in SAH rats.There is no evident correlation between the level of ADMA in plasma and the degree of CVS.
出处
《中华实验外科杂志》
CAS
CSCD
北大核心
2013年第12期2618-2621,共4页
Chinese Journal of Experimental Surgery
基金
四川省医学重点建设科学基金资助项目[川卫办发（2007）407]
四川省教育厅科研资助课题项目（13ZB0237）
川北医学院科研发展计划项目（CBY12-A-ZD12）
