摘要
目的观察斑蝥素及其衍生物去甲斑蝥素对人肝癌甲胎蛋白(alpha fetoprotein,AFP)阴性表达细胞系SK-HEP-1及AFP阳性表达肝癌细胞系HepG2凋亡的影响。方法采用四氮甲基唑蓝比色法分析斑蝥素、去甲斑蝥素对肝癌细胞生长的抑制作用,采用平板克隆形成实验检测斑蝥素、去甲斑蝥素对肝癌细胞克隆形成能力的影响,采用流式细胞仪检测细胞周期与凋亡情况。结果斑蝥素及去甲斑蝥素对SK-HEP-1及HepG2的生长均有抑制作用,且呈明显剂量和时间依赖效应;流式细胞仪检测结果提示,斑蝥素及去甲斑蝥素作用SK-HEP-1及HepG2后明显抑制细胞周期,呈G2/M期阻滞,并可诱导细胞凋亡。结论斑蝥素及去甲斑蝥素可能参与抑制肝癌细胞增殖、诱导细胞凋亡,有望成为肝癌有效治疗药物。
Objective To explore the effect of cantharidin and its derivatives, norcantharidin, on the apoptosis of SK- HEPq, the negative expression of alpha fetoprotein, and HepG2, the positive expression of alpha fetoprotein. Methods The rate of hepatoma cells growth inhibition was measured by MTT method. Clone formation ability was determined by flat plate clone formation assay. Flow cytometry was used to measure the cell cycle and apoptosis. Results Cantharidin and norcantharidin efficiently inhibited the growth of SK-HEP-1 and HepGz in a time- and dose- dependent manner. The flow cytometric profiles revealed that cantharidin and norcantharidin caused G2/M cell-cycle arrest in hepatoma cells, and induced the apoptosis after they worked on SK-HEP-1 and HepG2. Conclusion Cantharidin and norcantharidin could inhibit hepatoma cells growth and induce cell apoptosis, and would be the effective drug for hepatic cancer.
出处
《中华实用诊断与治疗杂志》
2013年第12期1159-1161,共3页
Journal of Chinese Practical Diagnosis and Therapy
基金
国家自然科学基金(81272542)
关键词
肝癌
斑蝥素
去甲斑蝥素
增殖
周期阻滞
凋亡
Hepatic cancer
cantharidin
norcantharidin
proliferation
cell cycle arrest
apoptosis
