摘要
采用高效液相色谱法，以AgilentEclipseXDB—C18柱（4．6mm×15cm，5μm）为色谱柱，甲醇-水-磷酸（55；45：0．2）为流动相，流速为1．0mL·min-1，检测波长为280nm，进样量为5～10μL，柱温为30℃．在给定的色谱条件下，黄芩苷与相邻峰分离度大于1．5，阴性对照试验无干扰．黄芩苷进样量在0．192～1．283μg之间与峰面积呈良好线性关系，回归方程Y=-0．3245＋53．295X，r=0．9998．平均加样回收率为101．3％，RSD=1．85％．该法操作简便，结果准确，重现性好，可用于消瘰颗粒剂的醇提取工艺研究及中间体质控．
To establish a method for determination of baicalin in dried ethanol extract of Xiaoluo granule, a HPLC method was set up. The sample was separated and analyzed with the Agilent Eclipse XDB--C18 column （4.6 mm× 15 cm, 5μm） as chromatogram column, and the methanol-- water-- phosphic acid solution （55 ： 45 ： 0.2） as mobile phase. The flow rate was set at 1.0 mL min-1 , the detective wavelength at 280 nm, the injection volume was 5-10 μL, and the column temperature was 30 ℃. With this chromatographic condition, the baicalin peak in dried ethanol extract sample of Xiaoluo granule chromatogram could be separated from other ingredient peaks completely. The calibration curve was linear at a ranges of 0. 192-1. 283μg with r = 0. 9998, and the average recovery from sample was 101.3% and RSD = 1.85%. This method is simple, accurate, re- producible, and can be used for extract preparation and intermediate quality control of Xiaoluo granule.
出处
《嘉兴学院学报》
2013年第6期117-120,共4页
Journal of Jiaxing University
基金
浙江省大学生科技活动计划项目(新苗计划)(2012R417033)
