摘要
目的:在成功建立目前公认的老年性痴呆(AD)体外模型基础上,观察补肾化痰益智法抗炎治疗AD的作用及机制。方法:运用Aβ1-42作用于BV-2细胞,同时用补肾化痰益智方含药血清进行干预,以吲哚美辛为对照。ELISA法检测细胞释放白介素-1β(IL-1β)、白介素-6(IL-6)、肿瘤坏死因子-α(TNF-α)的情况,RT-PCR法检测IL-1βmRNA的表达。结果:补肾化痰益智组与吲哚美辛组比较,能够更有效抑制IL-1β的表达(P<0.05),并能够从基因水平调节IL-1βmRNA的表达,从而抑制BV-2细胞分泌炎性因子的作用。结论:Aβ神经毒性与AD免疫炎性反应具有相关性。补肾化痰益智法能够调控炎性细胞因子IL-1β、IL-6、TNF-α及IL-1βmRNA的表达,从基因水平抑制炎性反应。
Objectives： Based on the successful establishment in vitro model of Alzheimer＇s disease （AD）, the effects and mechanism on anti-inflammatory of AD was investigated by Bushen Human Yizhi Recipe （BSHTYZR）. Methods： BV-2 cells effected by Aβ1-42 were produced for microglia model of AD, and then intervened by the serum containing-BSHTYZR, which was compared to the serum containing-Indometacin Enteric-Tablets （Indometacin）. The protein expression of IL-1β, IL-6, TNF-α was examined by ELISA, and IL-1β mRNA was detected by RT-PCR. Results： The BSHTYZ group could more effectively inhibit the expression of IL-1β （P〈0.05）. BSHTYZ Method could regulate the expression of IL-1β mRNA from the level of gene, then to suppress the secretion of inflammatory factors on BV-2 cells. Conclusion： There is a relationship between Aβ neurotoxicity and immune inflammatory response in AD. BSHTYZ method can regulate inflammatory cytokine IL-1β, IL-6, TNF-u and IL-1β mRNA＇s expression, and inhibit the inflammatory response in AD from the genetic level.
出处
《中华中医药杂志》
CAS
CSCD
北大核心
2013年第12期3694-3697,共4页
China Journal of Traditional Chinese Medicine and Pharmacy
基金
国家自然科学基金重点项目(No.81130064)
高等学校博士学科点专项科研基金项目(No.20094230110001)~~
关键词
补肾化痰益智法
老年性痴呆
BV-2细胞
炎性细胞因子
Therapy of replenishing kidney-essence and removing phlegm to benefit wisdom （Bushen Huatan Yizhi method）
Alzheimer＇s disease
BV-2
Inflammatory cytokines
