摘要
为获得春兰的SRAP标记图谱,对春兰SRAP-PCR反应体系进行了初步研究,通过正交试验设计,从Mg2+、dNTPs、Taq酶、引物、模板5种因素4个水平对春兰SRAP-PCR反应体系进行优化。所建立的体系为:25μL反应体系中含有2.5 mmol/L的Mg2+,2 mmol/L的dNTP,0.5 U的Taq酶,1.2μmol/L的引物,90 ng的模板。本研究为春兰指纹图谱的构建奠定了基础。
To obtain Sequence-related amplified polymorphism （SRAP） fingerprints of Cymbidium goeringii, the factors influencing the SRAP reaction system were studied. The major components of SRAP, such as concentrations of Mg2＊, dNTP, Taq DNA polymerase, primers and template, were optimized in this study by orthogonal design in five factors four levels respectively. The results showed that the optimum SRAP reaction system included Mg2＋ 2.5 mmol/L, dNTP 2 mmol/L, Taq DNApolymerase 0.5 U, primer 1.2 mmol/L and DNA template 90 ng in the total 25 p,L reaction system. It laid the foundation for the construction of fingerprints
出处
《农学学报》
2013年第11期25-29,共5页
Journal of Agriculture
基金
国家公益专项"国兰新品种培育与产业化生产关键技术研究"(201004080)
关键词
春兰
SRAP
正交试验
优化
Cymbidium goeringii
SRAP
Orthogonal Design
Optimization
